- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-TGF beta 1 antibody
See all TGF beta 1 primary antibodies -
DescriptionChicken polyclonal to TGF beta 1
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Host speciesChicken
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SpecificityFull length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
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Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
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Species reactivityReacts with: Mouse, Rat, Human, Pig
Predicted to work with: a wide range of other species
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Immunogen
Synthetic peptide:
RKPKVEQLSNMIVRS
, corresponding to amino acids 372-386 of Human and Mouse TGF beta 1. -
Positive control
- Purchase matching WB positive control:Recombinant human TGF beta 1 protein
- This antibody gave a positive result when used in the following methanol fixed cell lines: T24/83
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... -
PurityIgY fraction
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ClonalityPolyclonal
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IsotypeIgY
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab46780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| WB | ||
| ELISA |
WB: 1/2000 - 1/5000. Predicted molecular weight: 47 kDa for the unprocessed precursor; 25 kDa for the mature protein; 13KDa for degraded or cleaved protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
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Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
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Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
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Sequence similaritiesBelongs to the TGF-beta family.
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Post-translational
modificationsGlycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
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Database links
- Entrez Gene: 7040 Human
- Entrez Gene: 21803 Mouse
- Entrez Gene: 59086 Rat
- Omim: 190180 Human
- SwissProt: P01137 Human
- SwissProt: P04202 Mouse
- SwissProt: P17246 Rat
- Unigene: 645227 Human
see all -
Alternative names
- Cartilage-inducing factor antibody
- CED antibody
- Differentiation inhibiting factor antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TGF beta 1 antibody (ab46780)ICC/IF image of ab46780 stained T24/83 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab46780 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- chicken (ab150173) IgY (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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Western blot - Anti-TGF beta 1 antibody (ab46780)
References
ab46780 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"