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Synthetic peptide corresponding to Human TGF beta 1. Surrounding amino acid 374.
Our Abpromise guarantee covers the use of ab92486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 44 kDa.
Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
|IHC-FrFl||Use at an assay dependent concentration. PubMed: 24647450|
|IHC-P||Use a concentration of 10 - 20 µg/ml.|
|IHC-Fr||Use a concentration of 10 - 20 µg/ml.|
Blocked using 5% milk TTBS.
Western blot of mouse 3T3 cell lysate with ab92486 at concentration 4 ug/ml.