Overview

  • Product nameTGF beta 1 Human ELISA Kit
    See all TGF beta 1 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 80 pg/ml
  • Range
    0.082 ng/ml - 60 ng/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.87 85% - 104%
    Serum 94.46 82% - 102%
    Plasma 95.78 93% - 103%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s TGF beta 1 (TGFB1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human TGF beta 1 in serum, plasma, cell culture supernatants.

    Many cells synthesize TGFβ1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.

    This assay employs an antibody specific for human TGF beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antihuman TGF beta 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

     

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Post-translational
    modifications
    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names
    • Camurati-Engelmann disease
    • CED
    • DPD1
    • LAP
    • Latency-associated peptide
    • TGF beta 1 protein
    • TGF-beta-1
    • TGFB
    • tgfb1
    • TGFB1_HUMAN
    • TGFbeta
    • Transforming growth factor beta 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

TGF beta 1 Human ELISA Kit images

  • Standard curve in Assay Diluent A : mean of duplicates (+/-SD)

  • Standard curve in Assay Diluent B : mean of duplicates (+/-SD)

Protocols

References for TGF beta 1 Human ELISA Kit (ab100647)

ab100647 has not yet been referenced specifically in any publications.

Product Wall

TGFb human macrophage and DC secretion

Excellent Excellent 5/5 (Ease of Use)
Abreviews
TGFb human macrophage & DC secretion in culture for 3, isolated by negative selection.
The assay is easy to use and takes about 5.30hr.
Good specificity as shown by TGFb secretion by different macrophages subtyes & DC.
M0 = MCSF M1 = MCSF+IFNg M2 = MCSF+IL4 DC = GMCSF+IL4.
Username

Abcam user community

Verified customer

Submitted May 02 2014

human TGF beta 1 detection in M6 cells

Excellent Good 4/5 (Ease of Use)
Abreviews
The Kit works without any remarkable problem.
It isn't a very consuming time protocol.
As control we use mouse cells supernatant and the result was very low.
Username

Abcam user community

Verified customer

Submitted Oct 23 2013

Thank you for contacting us. Yes, you can store the HCl and the Urea/Acetic acid for later use, you do not have to use the diluted versions all at once. Please let me know if you have any further questions.



Avec ab100647, nous recommandons d’activer vos échantillons en faisant une extraction à l'acide, voir page 9 -10 du protocole.

Il est fortement recommandé d’effectuer cette l'extraction à l'a...

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Both antibodies are produced in Rat and monoclonal.

The laboratory that developed the ELISA did not send data showing results +/- acid treatment but did send the following summary. Please let me know if you still have questions or concerns.

It is strongly recommended that the customer perform...

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Thank you for contacting Abcam regarding ab100647.

I have received some further information from the lab regarding this issue and I wanted to share this with you.

The appearance of Assay Diluent B can vary by batch but a little cl...

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Thank you again for your call yesterday, and I just want to follow-up to make sure your experimentwent smoothly.

Please keep me updated about the results using the modified diluent, and let me know if you have any questions or if there is any...

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Thank you for calling Abcam earlier today.
I have talked to the lab and they have told me that Assay Diluent B does contain some protein and solutes so it would not be uncommon to see a little cloudiness or floating unsolubilized protein aggregate...

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Thank yhou for your enquiry.

I have copied some information belowproviding tips for sample preparation when using this kit, which includes human serum prepration.

I hope this will be helpful to you. if you have any further questio...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"