Overview

  • Product nameTGF beta 1 Mouse ELISA KitSee all TGF beta 1 kits ...
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4 7.9%
    Overall 4 7.9%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    7.8 pg/ml
  • Range
    31.3 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 124% - 141%
    Serum 92% - 103%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s TGF beta 1 Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Mouse TGF beta 1 concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate).

    Mouse TGF beta 1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Mouse TGF beta 1 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mouse TGF beta 1 captured on the plate

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Post-translational
    modifications
    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names
    • Camurati-Engelmann disease
    • CED
    • DPD1
    • LAP
    • Latency-associated peptide
    • TGF beta 1 protein
    • TGF-beta-1
    • TGFB
    • tgfb1
    • TGFB1_HUMAN
    • TGFbeta
    • Transforming growth factor beta 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

TGF beta 1 Mouse ELISA Kit images

  • Representative Standard Curve using ab119557.

Protocols

References for TGF beta 1 Mouse ELISA Kit (ab119557)

This product has been referenced in:
  • Fei AH  et al. Salvianolate inhibits reactive oxygen species production in H(2)O(2)-treated mouse cardiomyocytes in vitro via the TGFß pathway. Acta Pharmacol Sin 34:496-500 (2013). Read more (PubMed: 23524570) »
  • Kim DW  et al. Complex temporal changes in TGFß oncogenic signaling drive thyroid carcinogenesis in a mouse model. Carcinogenesis 34:2389-2400 (2013). Read more (PubMed: 23698635) »

See all 2 Publications for this product

Product Wall

Cross-reactivity with human TGF-b1

Inconclusive Average 3/5 (Ease of Use)
Abreviews
As we plan to do TGF-b1 determinations from supernatants of human and mice co-cultured cells, we checked the ability of this Kit to distinguish both forms, human and rodent.

We measured samples of DMEM, DMEM 10% and 0,5% FBS and DMEM + purified hTGF-b1 protein. Although samples with low FBS gives us a low value of TGF, samples with hTGF-b1 gives very strong signal.

We consider that specificity of the kit is not promised, so we want a discount check for the value of the Kit. Also we want to notice that we buy two different Kit to be absolute sure that the Kit is the problem not our manipulation, so we claim for both Kits...



Despite of the Kit is very easy to use, we consider that detection is a long process due to a lot steps of washing comparing to hTGFb1 detection Kit.
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Abcam user community

Verified customer

Submitted Oct 28 2013


ab108792: We have never tested brain tissue supernatants so I do not know if it would work. I cannot recommend the dilution range.

ab119557: Again, we have not tested brain tissue with this kit but there is a publication where a FlowCyto...

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We do have reports concerning tissue samples for ab119557 indicating that lysis buffers containing 0.5% TritonX-100 (detergent) had no deleterious effect, but we did not test this ourselves.


We also have not tes...

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Thank you for contacting us.

The TGF beta 1 Mouse ELISA Kit (with color giving dyes) (ab119557) is the only kit we currently have which can be used to detect TGF beta 1 in mouse samples. Regrettably we have not as yet tested this kit with tis...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"