TGF beta 1 Mouse ELISA Kit (ab119557)
- Product nameTGF beta 1 Mouse ELISA KitSee all TGF beta 1 kits ...
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Overall 7.9% Overall 7.9% Inter-assay Sample n Mean SD CV% Overall 5.8%
- Tests1 x 96 test
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich (quantitative)
- Sensitivity7.8 pg/ml
- Range31.3 pg/ml - 2000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 124% - 141% Serum 92% - 103%
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Mouse
- Product overview
Abcam’s TGF beta 1 Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Mouse TGF beta 1 concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate).
This assay employs a monoclonal antibody for mouse TGF beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and resistin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated monoclonal anti-mouse TGF beta 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 6 units Biotin-Conjugate anti-Mouse TGF beta 1 monoclonal antibody 1 x 120µl Blue-Dye 1 x 400µl Green-Dye 1 x 400µl Hydrochloric acid 1 x 3ml Microplate coated with monoclonal antibody to mouse TGF beta 1 (12 x 8 wells) 1 unit Mouse TGF beta 1 Standard lyophilized (4 ng/mL upon reconstitution) 2 vials Red-Dye 1 x 400µl Sodium hydroxide 1 x 3ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml
- FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
- Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
- Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
- Sequence similaritiesBelongs to the TGF-beta family.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
- Cellular localizationSecreted > extracellular space > extracellular matrix.
- Camurati-Engelmann disease
- Latency-associated peptide
- TGF beta 1 protein
- Transforming growth factor beta 1
Our Abpromise guarantee covers the use of ab119557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for TGF beta 1 Mouse ELISA Kit (ab119557)
This product has been referenced in:
- Fei AH et al. Salvianolate inhibits reactive oxygen species production in H(2)O(2)-treated mouse cardiomyocytes in vitro via the TGFß pathway. Acta Pharmacol Sin 34:496-500 (2013). Read more (PubMed: 23524570) »
- Kim DW et al. Complex temporal changes in TGFß oncogenic signaling drive thyroid carcinogenesis in a mouse model. Carcinogenesis 34:2389-2400 (2013). Read more (PubMed: 23698635) »