• Product name
  • Description
    Mouse monoclonal to TGF beta 2
  • Specificity
    Ab36495 recognizes TGF beta 2. It does not recognize TGF beta 1 or TGF beta 3.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, IHC-Frmore details
    Unsuitable for: Neut
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Human TGF beta 2 aa 352-377. Immunogen sequence is identical to amino acids 50 -75 of mature human TGF beta 2


    Database link: P61812

  • Epitope
    Recognizes an epitope within amino acids 50-75 of the mature human TGF beta 2 protein.
  • General notes

    This antibody does not inhibit the binding of TGF beta 2 to the TGF beta 2 Type 2 Receptor in mink lung epithelial cells.



Our Abpromise guarantee covers the use of ab36495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 50 kDa.
IHC-Fr 1/10 - 1/50.
  • Application notes
    Is unsuitable for Neut.
  • Target


    • ab36495 at 1/1000 staining mouse kidney tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step was perfomred in citrate buffer. The tissue was then blocked and incubated with the antibody for 1 hour. A biotinylated rabbit anti-mouse antibody was used as the secondary.

      See Abreview

    • ab36495 staining GF beta 2 in Human MCF7 cells by Immunocytochemistry/ Immunofluorescence.

      Cells were fixed with formaldehyde, permeabilized with 0.1% Tween/TBS and blocked with 10% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 in 10% BSA/TBST) for 1 hour. An AlexaFluor®488-conjugated goat anti-mouse monoclonal IgG (1/250) was used as the secondary antibody.

      See Abreview


    This product has been referenced in:
    • Liu B  et al. Expressions of TGF-ß2, bFGF and ICAM-1 in lens epithelial cells of complicated cataract with silicone oil tamponade. Int J Ophthalmol 10:1034-1039 (2017). Read more (PubMed: 28730103) »
    • Guo W  et al. The MicroRNA 29 Family Promotes Type II Cell Differentiation in Developing Lung. Mol Cell Biol 36:2141 (2016). Read more (PubMed: 27215389) »

    See all 16 Publications for this product

    Customer reviews and Q&As

    I learned from the lab is that the antibody was validated on purified recombinant TGF-beta 2, and there is no unusual protocol requirement for getting good western blot results.

    It is possible that your samples just have low amounts of the p...

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    Thank you for contacting us.

    TGF beta 1 is often observed at about 12 kDa on blots, since the 48 kDa precursor is processed to a 12 kDa. I suspect that the band you observe between the two lower molecular weight markers is the 12 kDa subunit...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunocytochemistry/ Immunofluorescence
    Human Cell (MCF-7 breast cancer)
    MCF-7 breast cancer
    Yes - 0.1 % Tween/TBS
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C

    孝子 赤松

    Verified customer

    Submitted Aug 28 2012

    Thank you for your inquiry.

    This antibody was tested in WB with recombinant TGFb2 which therefore can be used as positive control. We also have a positive Abreview from a customer which used mouse kidney and had veryclear positive staining. <...

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    Thank you for your enquiry.

    ab9758 - the major band at44.3 kDa corresponding to the unprocessed (pre-pro-TGF beta 1) form, the mature processed TGF beta 1 is a 25 kD homodimer composed of two 12.5 kDa subunits joined by disulfide bonds. As th...

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    Here is the MSDS as requested.

    It can also now be accessed from the datasheet of ab36495.

    I hope this has been of help. If you have any further questions or requests please do not hesitate to contact us again.

    Thank you for contacting us.

    We usually present the MSDS documents to the right hand side of the datasheet of products. The product you are interested in (ab36495) does not have an MSDS presented as it does not contain any materials whi...

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    Thank you for your enquiry.
    Yes, you are correct that ab36495, although it is a mouse antibody, can recognize mouse TGF beta-2. Here is a review from a customer who tested ab36495 for reactivity to mouse tissue: http://www.abcam.com/index.html?dat...

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    DISCOUNT CODE: xxxxxx; Expiration date: xxxxxxx I am very pleased to hear you would like to accept our offer and test ab36495 in IHC-GMA. This code will give you 1 free before the expiration date. To redeem this offer, please submit an Abrevi...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Blocking step
    BSA as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C

    Mr. Daniel Bird

    Verified customer

    Submitted Aug 17 2007


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