Our Abpromise guarantee covers the use of ab66045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.


Anti-TGF beta 2 antibody images

  • Human placenta stained with Anti-TGF beta 2 antibody (ab66045) at 1/50.
  • All lanes : Anti-TGF beta 2 antibody (ab66045) at 1/500 dilution

    Lane 1 : Rat Placenta
    Lane 2 : Rat Skeletal Muscle Negative Control

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Whole Tissue Lysate from Rat. An immunoreactive band of approx. 50kDa is observed in rat placenta but not in muscule.
  • ICC/IF image of ab66045 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66045, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-TGF beta 2 antibody (ab66045)

This product has been referenced in:
  • Wu Z  et al. RNAi-mediated silencing of AQP1 expression inhibited the proliferation, invasion and tumorigenesis of osteosarcoma cells. Cancer Biol Ther 16:1332-40 (2015). WB ; Human . Read more (PubMed: 26176849) »
  • Bettahi I  et al. Genome-wide transcriptional analysis of differentially expressed genes in diabetic, healing corneal epithelial cells: hyperglycemia-suppressed TGFß3 expression contributes to the delay of epithelial wound healing in diabetic corneas. Diabetes 63:715-27 (2014). IHC ; Rat . Read more (PubMed: 24306208) »

See all 4 Publications for this product

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I was able to get this WB testing data from the lab (attached). I've asked our datasheets team to add the image to our online datasheet. This is the caption that goes along with the image:...

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Thank you for contacting Abcam regarding ab66045.

I am sorry that the caption describing the WB image was confusing. Please allow me to clarify:

The image represents a WB in which the antibody ab66045 was used to detect ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Kidney)
Specification Kidney
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

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Submitted Jun 01 2011