Overview

  • Product nameAnti-TGF beta antibody
    See all TGF beta primary antibodies
  • Description
    Rabbit polyclonal to TGF beta
  • Tested applicationsSuitable for: WB, IP, ELISA, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide (17 aa) derived from C terminal human TGF beta 3 protein. (Peptide available as ab119811.)

  • Positive control
    • Breast carcinomas (MCF-7), Human Placenta, Intestine.

Properties

Applications

Our Abpromise guarantee covers the use of ab66043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Post-translational
    modifications
    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARVD antibody
    • BSC 1 cell growth inhibitor antibody
    • CED antibody
    • Cetermin antibody
    • DPD1 antibody
    • G TSF antibody
    • Glioblastoma derived T cell suppressor factor antibody
    • LAP antibody
    • Latency-associated peptide antibody
    • Polyergin antibody
    • TGF beta 1 protein antibody
    • TGF beta 3 antibody
    • TGF beta1 antibody
    • TGF beta2 antibody
    • TGF beta3 antibody
    • TGF-beta-1 antibody
    • TGFB antibody
    • tgfb1 antibody
    • TGFB1_HUMAN antibody
    • TGFB2 antibody
    • TGFB3 antibody
    • TGFbeta antibody
    • Transforming growth factor antibody
    • Transforming growth factor beta 1 antibody
    • Transforming growth factor beta 2 antibody
    • Transforming growth factor beta 3 antibody
    see all

Anti-TGF beta antibody images

  • ab66043 staining TGF beta in Human BAL cell tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with HOPE and blocked with 100% blocking solution for 5 minutes at 25°C. Samples were incubated with primary antibody (1/200 in blocking buffer) for 1 hour at 25°C. A  HRP-conjugated Rat IgG polyclonal was used as the secondary antibody.

  • ab66043 staining TGF beta in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% TritonX100 in PBS. Samples were incubated with primary antibody (1/1000 in PBS) for 1 hour at 22°C. ab150081 was used as the secondary antibody at 1/200.

  • ab66043 staining TGF beta in Rat liver tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 0.25% serum for 15 minutes at 25°C ; antigen retrieval was by heat mediation in citrate (pH 6.0) buffer. Samples were incubated with primary antibody (1/50 in serum free antibody diluent) for 18 hours at 4°C. An undiluted HRP-conjugated Goat polymer was used as the secondary antibody.

    See Abreview

  • Anti-TGF beta antibody (ab66043) at 1/500 dilution (for 18 hours at 4°C) + Whole tissue lysate of mouse brain at 20 µg

    Secondary
    An HRP-conjugated Goat polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 13 kDa
    Observed band size : 13 kDa


    Exposure time : 90 seconds

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at 20°C

    See Abreview

  • Human placenta stained with Anti-TGF beta antibody (ab66043) at 1/100.
  • Anti-TGF beta antibody (ab66043) at 1/500 dilution + whole tissue lysate prepared from rat intestine at 15 µg

    Secondary
    Goat anti-rabbit conjugated to HRP at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 13 kDa
    Observed band size : 13 kDa


    Exposure time : 2 minutes

    Image courtesy of an anonymous Abreview.

    Primary antibody incubated for 2 hours at 23°C.
    Blocking step performed using 5% milk for 1 hour at 23°C.

    See Abreview

  • ICC/IF image of ab66043 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66043, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-TGF beta antibody (ab66043)

This product has been referenced in:
  • Quan FS  et al. Comparative effect of immature neuronal or glial cell transplantation on motor functional recovery following experimental traumatic brain injury in rats. Exp Ther Med 12:1671-1680 (2016). Read more (PubMed: 27602084) »
  • Furukawa J  et al. GLI2 expression levels in radical nephrectomy specimens as a predictor of disease progression in patients with metastatic clear cell renal cell carcinoma following treatment with sunitinib. Mol Clin Oncol 5:186-192 (2016). Read more (PubMed: 27602218) »

See all 26 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Bone)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TE pH8
Permeabilization No
Specification Bone
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative 10% normal buffered formalin
Username

Abcam user community

Verified customer

Submitted Aug 26 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Jaculus jaculus Tissue sections (Metatarsals, chondrocytes)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization No
Specification Metatarsals, chondrocytes
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 15°C
Fixative Paraformaldehyde
Username

HAYDEE GUTIERREZ

Verified customer

Submitted Jun 08 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.5% TritonX100 in PBS
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Aug 07 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lung cancer)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 30min
Permeabilization No
Specification lung cancer
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 05 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Antigen retrieval step None
Sample Mouse Tissue sections (Spinal cord)
Specification Spinal cord
Permeabilization Yes - Triton
Fixative Paraformaldehyde
Username

Andrew Greenhalgh

Verified customer

Submitted Dec 03 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (13%)
Sample Mouse Cell lysate - whole cell (Liver tissue)
Specification Liver tissue
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Suypriya Sen

Verified customer

Submitted May 13 2014

Thank you for contacting us.

Yes, this is a pan antibody. Even though the immunogen was taken from TGF beta 3, the antibody reacts with TGF beta 1, 2, and 3.


I hope this information is helpful to you. Please do not hesitate ...

Read More
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Tissue lysate - whole (Cardiac Muscle)
Specification Cardiac Muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 27 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12% Bis-Tris)
Sample Human Tissue lysate - whole (Skeletal Muscle (Gastrocnemius))
Specification Skeletal Muscle (Gastrocnemius)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris (pH8) better than Citrate (pH6)
Sample Human Tissue sections (Skeletal Muscle (Gastrocnemius))
Specification Skeletal Muscle (Gastrocnemius)
Permeabilization No
Fixative Methacarn
Username

Abcam user community

Verified customer

Submitted Aug 26 2013

1-10 of 67 Abreviews or Q&A

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