Recombinant
RabMAb

Anti-TGF beta induced factor 2 antibody [EPR5655(2)] (ab155948)

Overview

  • Product name
    Anti-TGF beta induced factor 2 antibody [EPR5655(2)]
    See all TGF beta induced factor 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR5655(2)] to TGF beta induced factor 2
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues within Human TGF beta induced factor 2 (UniProt Q9GZN2).

  • Positive control
    • Human fetal kidney tissue lysate; HeLa cell lysate; Human ovary cancer tissue lysate; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab155948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Transcriptional repressor, which probably repress transcription by binding directly the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins. Probably represses transcription via the recruitment of histone deacetylase proteins.
    • Tissue specificity
      Widely expressed. Highly expressed in heart, kidney and testis. Weakly expressed in brain and prostate.
    • Sequence similarities
      Belongs to the TALE/TGIF homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Post-translational
      modifications
      The C-terminal part is phosphorylated in response to EGF signaling by the Ras/MAPK pathway.
    • Cellular localization
      Nucleus. Excluded from nucleoli.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5' TG 3' interacting factor 2 antibody
      • 5''-TG-3''-interacting factor 2 antibody
      • Homeobox protein TGIF2 antibody
      • TGF (beta) induced transcription factor 2 antibody
      • TGF-beta-induced transcription factor 2 antibody
      • TGFB induced factor 2 antibody
      • TGFB-induced factor 2 antibody
      • Tgif2 antibody
      • TGIF2_HUMAN antibody
      • Transforming growth factor beta induced factor 2 antibody
      see all

    Images

    • All lanes : Anti-TGF beta induced factor 2 antibody [EPR5655(2)] (ab155948) at 1/1000 dilution

      Lane 1 : Human fetal kidney tissue lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Human ovary cancer tissue lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 26 kDa
    • Immunofluorescence analysis of HeLa cells, labeling TGF beta induced factor 2 using ab155948 at 1/250 dilution.

    References

    This product has been referenced in:
    • Hamabe A  et al. Role of pyruvate kinase M2 in transcriptional regulation leading to epithelial-mesenchymal transition. Proc Natl Acad Sci U S A 111:15526-31 (2014). Read more (PubMed: 25313085) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Melanoma cells 4L)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    60 µg
    Specification
    Melanoma cells 4L
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted May 15 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    ChIP
    Sample
    Human Cell lysate - whole cell (melanoma cell lines 4L and SkMel85)
    Specification
    melanoma cell lines 4L and SkMel85
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: para formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 04 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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