Anti-TGF beta Receptor III antibody [MM0057-5G9] (ab78421)

Overview

  • Product name
    Anti-TGF beta Receptor III antibody [MM0057-5G9]
    See all TGF beta Receptor III primary antibodies
  • Description
    Mouse monoclonal [MM0057-5G9] to TGF beta Receptor III
  • Specificity
    This antibody was selected for its ability to detect TGF beta Receptor III. No cross reactivity was found to TGF beta Receptor I and Receptor II.
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human TGF beta Receptor III extracellular domain

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate buffered saline (PBS).
  • Clonality
    Monoclonal
  • Clone number
    MM0057-5G9
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 93 kDa.
IHC-P 1/50 - 1/100.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Binds to TGF-beta. Could be involved in capturing and retaining TGF-beta for presentation to the signaling receptors.
  • Sequence similarities
    Contains 1 ZP domain.
  • Post-translational
    modifications
    Extensively modified by glycosaminoglycan groups (GAG).
  • Cellular localization
    Secreted. Secreted > extracellular space. Cell membrane. Exists both as a membrane-bound form and as soluble form in serum and in the extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta glycan antibody
    • Betaglycan antibody
    • Betaglycan proteoglycan antibody
    • BGCAN antibody
    • TGBR3_HUMAN antibody
    • TGF beta receptor type 3 antibody
    • TGF beta receptor type III antibody
    • TGF-beta receptor type 3 antibody
    • TGF-beta receptor type III antibody
    • TGFB R3 antibody
    • TGFBR 3 antibody
    • TGFBR3 antibody
    • TGFR 3 antibody
    • TGFR-3 antibody
    • TGFR3 antibody
    • Transforming growth factor beta receptor III antibody
    • Transforming growth factor beta receptor III betaglycan 300kDa antibody
    • Transforming growth factor beta receptor type 3 antibody
    see all

Images

  • ab78421 at 1/100 dilution, staining TGF beta Receptor III in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

  • Overlay histogram showing K562 cells stained with ab78421 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78421, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References

This product has been referenced in:
  • Yumoto K  et al. Axl is required for TGF-ß2-induced dormancy of prostate cancer cells in the bone marrow. Sci Rep 6:36520 (2016). Human . Read more (PubMed: 27819283) »
  • Liu G  et al. Dendritic cell SIRT1-HIF1a axis programs the differentiation of CD4+ T cells through IL-12 and TGF-ß1. Proc Natl Acad Sci U S A 112:E957-65 (2015). Read more (PubMed: 25730867) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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