Anti-TGF beta Receptor III/TGFBR3 antibody [MM0057-5G9] (ab78421)
Key features and details
- Mouse monoclonal [MM0057-5G9] to TGF beta Receptor III/TGFBR3
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TGF beta Receptor III/TGFBR3 antibody [MM0057-5G9]
See all TGF beta Receptor III/TGFBR3 primary antibodies -
Description
Mouse monoclonal [MM0057-5G9] to TGF beta Receptor III/TGFBR3 -
Host species
Mouse -
Specificity
This antibody was selected for its ability to detect TGF beta Receptor III/TGFBR3. No cross reactivity was found to TGF beta Receptor I and Receptor II.
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Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate buffered saline (PBS). -
Clonality
Monoclonal -
Clone number
MM0057-5G9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-P
1/50 - 1/100. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Binds to TGF-beta. Could be involved in capturing and retaining TGF-beta for presentation to the signaling receptors. -
Sequence similarities
Contains 1 ZP domain. -
Post-translational
modificationsExtensively modified by glycosaminoglycan groups (GAG). -
Cellular localization
Secreted. Secreted > extracellular space. Cell membrane. Exists both as a membrane-bound form and as soluble form in serum and in the extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 7049 Human
- Omim: 600742 Human
- SwissProt: Q03167 Human
- Unigene: 482390 Human
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Alternative names
- Beta glycan antibody
- Betaglycan antibody
- Betaglycan proteoglycan antibody
see all
Images
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ab78421 at 1/100 dilution, staining TGF beta Receptor III/TGFBR3 in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
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Overlay histogram showing K562 cells stained with ab78421 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78421, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab78421 has been referenced in 8 publications.
- Schilpp C et al. TGF-ß1 increases permeability of ciliated airway epithelia via redistribution of claudin 3 from tight junction into cell nuclei. Pflugers Arch 473:287-311 (2021). PubMed: 33386991
- Yue Y et al. SNHG5 protects chondrocytes in interleukin-1β-stimulated osteoarthritis via regulating miR-181a-5p/TGFBR3 axis. J Biochem Mol Toxicol 35:e22866 (2021). PubMed: 34369033
- Lin J et al. Berberine, a Traditional Chinese Medicine, Reduces Inflammation in Adipose Tissue, Polarizes M2 Macrophages, and Increases Energy Expenditure in Mice Fed a High-Fat Diet. Med Sci Monit 25:87-97 (2019). PubMed: 30606998
- Cornell L et al. MicroRNA-Mediated Suppression of the TGF-ß Pathway Confers Transmissible and Reversible CDK4/6 Inhibitor Resistance. Cell Rep 26:2667-2680.e7 (2019). PubMed: 30840889
- Han YL et al. Downregulation of microRNA-193-3p inhibits the progression of intrahepatic cholangiocarcinoma cells by upregulating TGFBR3. Exp Ther Med 15:4508-4514 (2018). PubMed: 29725386
- Bronevetsky Y et al. Lin28b Regulates Fetal Regulatory T Cell Differentiation through Modulation of TGF-β Signaling. J Immunol 197:4344-4350 (2016). PubMed: 27793996
- Yumoto K et al. Axl is required for TGF-ß2-induced dormancy of prostate cancer cells in the bone marrow. Sci Rep 6:36520 (2016). Human . PubMed: 27819283
- Liu G et al. Dendritic cell SIRT1-HIF1a axis programs the differentiation of CD4+ T cells through IL-12 and TGF-ß1. Proc Natl Acad Sci U S A 112:E957-65 (2015). PubMed: 25730867