• Product name
  • Description
    Rabbit polyclonal to TGFBI
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig
  • Immunogen

    A region within synthetic peptide: AEPDIMATNG VVHVITNVLQ PPANRPQERG DELADSALEI FKQASAFSRA, corresponding to C terminal amino acids 613-662 of Human TGFBI

  • Positive control
    • Jurkat cell lysate



Our Abpromise guarantee covers the use of ab66957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.


  • Function
    Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation.
  • Tissue specificity
    Highly expressed in the corneal epithelium.
  • Involvement in disease
    Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD) [MIM:121820]; also known as Cogan corneal dystrophy or map-dot-fingerprint type corneal dystrophy. EBMD is a bilateral anterior corneal dystrophy characterized by grayish epithelial fingerprint lines, geographic map-like lines, and dots (or microcysts) on slit-lamp examination. Pathologic studies show abnormal, redundant basement membrane and intraepithelial lacunae filled with cellular debris. Although this disorder usually is not considered to be inherited, families with autosomal dominant inheritance have been identified.
    Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1) [MIM:121900]; also known as corneal dystrophy granular type. Inheritance is autosomal dominant. Corneal dystrophies show progressive opacification of the cornea leading to severe visual handicap.
    Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1) [MIM:122200]. Inheritance is autosomal dominant.
    Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB) [MIM:602082]; also known as corneal dystrophy of Bowman layer type 2 (CDB2).
    Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB) [MIM:608470]; also known as corneal dystrophy of Bowman layer type 1 (CDB1).
    Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A) [MIM:608471]. CDL3A clinically resembles to lattice corneal dystrophy type 3, but differs in that its age of onset is 70 to 90 years. It has an autosomal dominant inheritance pattern.
    Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD) [MIM:607541]. ACD could be considered a variant of granular dystrophy with a significant amyloidogenic tendency. Inheritance is autosomal dominant.
  • Sequence similarities
    Contains 1 EMI domain.
    Contains 4 FAS1 domains.
  • Post-translational
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix. May be associated both with microfibrils and with the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • RGD containing collagen associated protein antibody
    • AI181842 antibody
    • AI747162 antibody
    • Beta ig antibody
    • Beta ig h3 antibody
    • Beta ig-h3 antibody
    • BGH3_HUMAN antibody
    • Big h3 antibody
    • BIGH3 antibody
    • CDB1 antibody
    • CDG2 antibody
    • CDGG1 antibody
    • CSD antibody
    • CSD1 antibody
    • CSD2 antibody
    • CSD3 antibody
    • EBMD antibody
    • Kerato epithelin antibody
    • Kerato-epithelin antibody
    • LCD1 antibody
    • MGC150270 antibody
    • RGD CAP antibody
    • RGD-CAP antibody
    • RGD-containing collagen-associated protein antibody
    • TGFBI antibody
    • TGFBI transforming growth factor, beta induced, 68kDa antibody
    • Transforming growth factor beta induced protein ig h3 antibody
    • Transforming growth factor-beta-induced protein ig-h3 antibody
    see all


  • All lanes : Anti-TGFBI antibody (ab66957) at 2.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.
  • Immunofluorescence analysis of Human corneal tissue staining TGFBI with ab66957.

  • Anti-TGFBI antibody (ab66957) at 1.25 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk / PBS buffer

    HRP conjugated anti-rabbit IgG at 50,000 - 100,000 dilution

    Observed band size: 46 kDa (why is the actual band size different from the predicted?)


This product has been referenced in:
  • Huang AH  et al. Evaluation of the complex transcriptional topography of mesenchymal stem cell chondrogenesis for cartilage tissue engineering. Tissue Eng Part A 16:2699-708 (2010). Read more (PubMed: 20367254) »

See 1 Publication for this product

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