Anti-TGIF antibody (ab220965)
Key features and details
- Rabbit polyclonal to TGIF
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TGIF antibody
See all TGIF primary antibodies -
Description
Rabbit polyclonal to TGIF -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Recombinant fragment corresponding to Human TGIF aa 250-400. Numbering is from isoform 1; the sequence also occurs in isoforms 2, 3 and 4.
Database link: Q15583 -
Positive control
- RT4 cell line; A549 cells
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General notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 43 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 43 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities. -
Involvement in disease
Holoprosencephaly 4 -
Sequence similarities
Belongs to the TALE/TGIF homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 455312 Chimpanzee
- Entrez Gene: 7050 Human
- Omim: 602630 Human
- SwissProt: Q5IS58 Chimpanzee
- SwissProt: Q15583 Human
- Unigene: 373550 Human
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Alternative names
- 5' TG 3' interacting factor antibody
- 5''-TG-3''-interacting factor 1 antibody
- Homeobox protein TGIF antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab220965 has been referenced in 1 publication.
- Kong L et al. TGIF1 plays a carcinogenic role in esophageal squamous cell carcinoma through the Wnt/ß-catenin and Akt/mTOR signaling pathways. Int J Mol Med 47:N/A (2021). PubMed: 33693954