Overview

  • Product nameAnti-TGN46 antibody [2F7.1]
    See all TGN46 primary antibodies
  • Description
    Mouse monoclonal [2F7.1] to TGN46
  • Tested applicationsSuitable for: IP, Inhibition Assay, IHC-Fr, ICC, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Hamster, Human, Non Human Primates, African Green Monkey, Chinese Hamster
  • Immunogen

    Synthetic peptide corresponding to Rat TGN46 aa 1-17.
    Sequence:

    LPSASKPNNTSSENNPP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityAscites
  • ClonalityMonoclonal
  • Clone number2F7.1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 22456512
ICC 1/20 - 1/50.
WB 1/500.

This antibody detects an ~38 kDa protein representing recombinant rat TGN38.

Flow Cyt 1/20 - 1/50.

Target

  • FunctionMay be involved in regulating membrane traffic to and from trans-Golgi network.
  • Tissue specificityIsoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.
  • Cellular localizationCell membrane. Golgi apparatus > trans-Golgi network membrane. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • TGN 46 antibody
    • TGN 51 antibody
    • TGN38 antibody
    • TGN38 homolog antibody
    • TGN46 antibody
    • TGN48 antibody
    • TGN51 antibody
    • TGOLN2 antibody
    • TGON2_HUMAN antibody
    • Trans Golgi network integral membrane protein 2 antibody
    • Trans golgi network protein 2 antibody
    • Trans Golgi network protein TGN51 antibody
    • Trans-Golgi network integral membrane protein 2 antibody
    • Trans-Golgi network protein TGN51 antibody
    • TTGN2 antibody
    see all

Anti-TGN46 antibody [2F7.1] images

  • NRK cells were stained with anti-TGN46 ab2809 (red) anti-beta-COP ab2899 (green) and DAPI.
  • Immunofluorescent analysis of Phalloidin (green) and TGN46(purple) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a TGN46 monoclonal antibody (ab2809) at a dilution of 1:75 for at least 1 hour at room temperature and incubated with DyLight 680 goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin at a dilution of 1:300 (1 unit/ml final concentration) for 30 minutes. Images were taken at 20X magnification.
  • HeLa and COS7 cells were rinsed with PBS and fixed with -20�C methanol for 5 minutes. Cells were incubated with anti-TGN46 antibody (ab2809) diluted 1:100 in PBS containing 0.2% BSA for 1 hour. The cells were then rinsed in PBS and incubated with goat anti-mouse IgG conjugated to rhodamine for 30 minutes in PBS containing 0.2% BSA.
  • ICC/IF image of ab2809 stained rat PC12 cells. The cells were 4% PFA and 100% Methanol fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2809, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
  • ab2809 staining Chinese Hamster Ovary cell by Immunocytochemistry/Immunofluorescence. The cells were paraformaldehyde fixed and permeabilized in 0.5% Triton prior to blocking with 10% FCS for 30 minutes at room temperature. The primary antibody was diluted 1/1000 and incubated with the sample for 2 hours. The secondary antibody was an Alexa Fluor® 488-conjugated Goat antibody, diluted 1/1000.

    See Abreview

  • Overlay histogram showing CHO cells stained with ab2809 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2809, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was goat anti-mouse DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in CHO cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-TGN46 antibody [2F7.1] (ab2809)

This product has been referenced in:
  • Zhang J  et al. Recombinant Murine Gamma Herpesvirus 68 Carrying KSHV G Protein-Coupled Receptor Induces Angiogenic Lesions in Mice. PLoS Pathog 11:e1005001 (2015). IF ; Human . Read more (PubMed: 26107716) »
  • Lengfeld J  et al. Protein kinase C d regulates the release of collagen type I from vascular smooth muscle cells via regulation of Cdc42. Mol Biol Cell 23:1955-63 (2012). IHC-Fr . Read more (PubMed: 22456512) »

See all 12 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (HepG2 cells)
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Loading amount 20 µg
Specification HepG2 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Oct 27 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HepG2)
Permeabilization Yes - 0.2% Triton X-100
Specification HepG2
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Oct 14 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat hepatocytes)
Permeabilization Yes - 0.2% Triton X-100
Specification Rat hepatocytes
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Aug 24 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Human Cell (fibroblasts)
Specification fibroblasts
Permeabilization Yes - 0.3% TritonX-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 21 2014

The only hamster data we have for ab2809 is a review we received from a customer who used it for staining CHO cells. Here is a link to the review:

http://www.abcam.com/TGN46-2F71-antibody-ab2809/reviews/21415

Based on this data, w...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (cultured hippocampal neurons)
Specification cultured hippocampal neurons
Fixative Formaldehyde
Permeabilization Yes - 0.2% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 18 2012

Thanks so much for your reply.

If you don't mind I will include your reply in the 'Additional notes' section of your review as I think your comment will be quite helpful for other customers.

Thanks again for the additional information.

Thank you for submitting your Abreview. As you have noted a 'Poor' rating I just wanted to follow up with you to get some further details as to why you selected this rating and to perhaps assist you in improving your results.

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample African Green Monkey Cell (COS7)
Specification COS7
Fixative Formaldehyde
Permeabilization Yes - 0.2% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 17 2012

Thank you for your enquiry.

The use of human cells in the review you mentioned is the only data I can find regarding use of this antibody to recognize TGN46. Actually there is some conflicting data regarding detection of human protein with th...

Read More

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"