Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use a concentration of 5 µg/ml.

Target

  • FunctionMay be involved in regulating membrane traffic to and from trans-Golgi network.
  • Tissue specificityIsoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.
  • Cellular localizationCell membrane. Golgi apparatus > trans-Golgi network membrane. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • TGN 46 antibody
    • TGN 51 antibody
    • TGN38 antibody
    • TGN38 homolog antibody
    • TGN46 antibody
    • TGN48 antibody
    • TGN51 antibody
    • TGOLN2 antibody
    • TGON2_HUMAN antibody
    • Trans Golgi network integral membrane protein 2 antibody
    • Trans golgi network protein 2 antibody
    • Trans Golgi network protein TGN51 antibody
    • Trans-Golgi network integral membrane protein 2 antibody
    • Trans-Golgi network protein TGN51 antibody
    • TTGN2 antibody
    see all

Anti-TGN46 antibody images

  • TGN46 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to TGN46 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16052.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 51kDa; TGN46

  • ICC/IF image of ab16052 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab16052, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • Image courtesy of Human Protein Atlas

    ab16052 staining TGN46 in Human appendix.The paraffin embedded human skin tissue was incubated with ab16052 (1/50 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab16052 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

    Further results for this antibody can be found at www.proteinatlas.org

  • Immunofluorescence analysis of SH-SY5Y cells transfected with CLU2, staining TGN46 with ab16052.

    Cells were fixed with ice-cold methanol and blocked with 5% goat serum. Samples were incubated with primary antibody (1/1000) overnight at 4°C. An AlexaFluor®568-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody.

References for Anti-TGN46 antibody (ab16052)

This product has been referenced in:
  • Elks CM  et al. Blueberries improve glucose tolerance without altering body composition in obese postmenopausal mice. Obesity (Silver Spring) 23:573-80 (2015). Read more (PubMed: 25611327) »
  • Greer AM  et al. Serum IgE clearance is facilitated by human FceRI internalization. J Clin Invest 124:1187-98 (2014). ICC/IF . Read more (PubMed: 24569373) »

See all 13 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample African Green Monkey Cultured Cells (COS7)
Specification COS7
Fixative Paraformaldehyde
Permeabilization Yes - 0.02% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Jul 09 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (hippocampal culture 14 DIV)
Specification hippocampal culture 14 DIV
Fixative Paraformaldehyde
Permeabilization Yes - 0.02%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

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Submitted Jul 06 2012

I am very pleased to hear you would like to accept our offer and test ab16052 in African Green Monkey. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forAfrican Green Monkeya...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (H1299)
Specification H1299
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 1 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted May 28 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK293 EBNA)
Specification HEK293 EBNA
Fixative 3% Paraformaldehyde
Permeabilization No
Blocking step NH4Cl as blocking agent for 15 minute(s) · Concentration: 0.27% · Temperature: 20°C
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Verified customer

Submitted Mar 31 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela)
Specification Hela
Fixative Formaldehyde
Permeabilization Yes - 0.1% saponin
Blocking step Casein block + 2.5% serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 21°C
Username

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Verified customer

Submitted Sep 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"