Overview

  • Product name
  • Description
    Rabbit polyclonal to TGN46
  • Specificity
    This antibody does not cross react with TGN46 - the human homologue of TGN46. A Rabbit polyclonal to human TGN46 is available (ab16052).
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chinese hamster
    Does not react with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Mouse TGN46.

    (Peptide available as ab16384.)

  • Positive control
    • ab16059 gave a positive result in the following whole cell lysates: NIH 3T3 (Mouse embryonic fibroblast cell line) MEF1 (Mouse embryonic fibroblast cell line) PC12 (Rat adrenal pheochromocytoma cell line)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 35 kDa).

Rodent TGN46 is extensively glycosylated and as a result has a mass of 97 kDa on an SDS-PAGE gel.

ICC/IF Use a concentration of 1 µg/ml.
ICC Use at an assay dependent concentration.

Target

  • Function
    May be involved in regulating membrane traffic to and from trans-Golgi network.
  • Tissue specificity
    Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.
  • Cellular localization
    Cell membrane. Golgi apparatus > trans-Golgi network membrane. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • TGN 46 antibody
    • TGN 51 antibody
    • TGN38 antibody
    • TGN38 homolog antibody
    • TGN46 antibody
    • TGN48 antibody
    • TGN51 antibody
    • TGOLN2 antibody
    • TGON2_HUMAN antibody
    • Trans Golgi network integral membrane protein 2 antibody
    • Trans golgi network protein 2 antibody
    • Trans Golgi network protein TGN51 antibody
    • Trans-Golgi network integral membrane protein 2 antibody
    • Trans-Golgi network protein TGN51 antibody
    • TTGN2 antibody
    see all

Anti-TGN46 antibody images

  • All lanes : Anti-TGN46 antibody (ab16059) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 35 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.

    Rodent TGN46 is extensively glycosylated and as a result has a mass of 97 kDa on an SDS-PAGE gel.

  • ICC/IF image of ab16059 stained mouse MEF1 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab16059, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • IHC image of ab16059 staining in mouse e14 whole foetus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16059, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-TGN46 antibody (ab16059)

This product has been referenced in:
  • Vural A  et al. Activator of G-Protein Signaling 3-Induced Lysosomal Biogenesis Limits Macrophage Intracellular Bacterial Infection. J Immunol 196:846-56 (2016). Read more (PubMed: 26667172) »
  • Oetjen S  et al. Revisiting the neuronal localization and trafficking of CLN3 in juvenile neuronal ceroid lipofuscinosis. J Neurochem 139:456-470 (2016). Read more (PubMed: 27453211) »

See all 12 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic Heart)
Permeabilization
Yes - Tween-20
Specification
Embryonic Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 05 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Rat Cell (Rat liver tissue section)
Specification
Rat liver tissue section
Permeabilization
Yes - PBS containing 0.25% Triton X-100
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Nov 18 2014


We initially tested ab16059 on human samples and did not detect any cross-reactivity with the human samples. Thus, the antibody should not detect human TGN46, and is tested and guaranteed to detect mouse, rat, and chinese hamster TGN46.

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
30 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Aug 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Chinese Hamster Cell (Chinese Hamster Ovary Cell)
Specification
Chinese Hamster Ovary Cell
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton
Blocking step
FCS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (keratinocytes)
Specification
keratinocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3%Triton in PBS
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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