Overview

  • Product nameAnti-THAP11 antibody
    See all THAP11 primary antibodies
  • Description
    Rabbit polyclonal to THAP11
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Recombinant Fragment
    Predicted to work with: Rat, Rabbit, Hamster, Cow, Human, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human THAP11.

  • Positive control
    • This antibody gave a positive signal in THAP11 recombinant protein as well as the following tissue lysates: Mouse Brain; Mouse Hippocampus.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).

Target

  • FunctionTranscriptional repressor that plays a central role for embryogenesis and the pluripotency of embryonic stem (ES) cells. Sequence-specific DNA-binding factor that represses gene expression in pluripotent ES cells by directly binding to key genetic loci and recruiting epigenetic modifiers.
  • Sequence similaritiesBelongs to the THAP11 family.
    Contains 1 THAP-type zinc finger.
  • Cellular localizationNucleus. Cytoplasm. May be regulated by shuttling of the protein between the cytoplasm and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CTG B43a antibody
    • CTG B45d antibody
    • HRIHFB2206 antibody
    • RONIN antibody
    • THA11_HUMAN antibody
    • THAP 11 antibody
    • THAP domain containing 11 antibody
    • THAP domain-containing protein 11 antibody
    • Thap11 antibody
    see all

Anti-THAP11 antibody images

  • All lanes : Anti-THAP11 antibody (ab104395) at 1 µg/ml

    Lane 1 : THAP11 Recombinant protein at 0.1 µg
    Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
    Lane 3 : Mouse Hippocampus Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 130 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References for Anti-THAP11 antibody (ab104395)

ab104395 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Cerebellum)
Specification Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Apr 30 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"