Overview

  • Product nameThapsigargin
  • Description
    Ca2+-ATPase inhibitor
  • Biological descriptionA potent, cell-permeable Ca2+-ATPase inhibitor. Releases Ca2+ by inhibiting endoplasmic reticular Ca2+-ATPase (IC50 = 4-13 nM). Both tumorogenic and apoptotic actions reported.
  • Purity> 98%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120286 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Thapsigargin images

    • ab58668 staining ATF3 in serum starved A549 cells treated with thapsigargin (ab120286), by ICC/IF. Increase of ATF3 correlates with increased concentration of thapsigargin, as described in literature.
      The cells were incubated at 37°C for 1h in media containing different concentrations of ab120286 (thapsigargin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab58668 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for Thapsigargin (ab120286)

    This product has been referenced in:
    • Treiman M  et al. A tool coming of age: thapsigargin as an inhibitor of sarco-endoplasmic reticulum Ca(2+)-ATPases. Trends Pharmacol Sci 19:131-5 (1998). Read more (PubMed: 9612087) »
    • Inesi G & Sagara Y Thapsigargin, a high affinity and global inhibitor of intracellular Ca2+ transport ATPases. Arch Biochem Biophys 298:313-7 (1992). Read more (PubMed: 1416963) »
    • Thastrup O  et al. Thapsigargin, a novel molecular probe for studying intracellular calcium release and storage. Agents Actions 27:17-23 (1989). Read more (PubMed: 2787587) »

    See all 3 Publications for this product

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