Anti-THBS4 antibody - N-terminal (ab211143)
Key features and details
- Rabbit polyclonal to THBS4 - N-terminal
- Suitable for: WB, IHC - Wholemount
- Reacts with: Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-THBS4 antibody - N-terminal
See all THBS4 primary antibodies -
Description
Rabbit polyclonal to THBS4 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC - Wholemountmore details -
Species reactivity
Reacts with: Zebrafish -
Immunogen
Recombinant fragment within Zebrafish THBS4 aa 2-342 (N terminal). The exact sequence is proprietary.
Database link: Q8JGW0 -
Positive control
- Zebrafish muscle whole cell lysate; 2 days-post-fertilization zebrafish embryo.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 104 kDa.
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IHC - Wholemount | (1) |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 104 kDa. |
IHC - Wholemount
1/100 - 1/500. |
Target
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Function
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen. -
Sequence similarities
Belongs to the thrombospondin family.
Contains 4 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 8 TSP type-3 repeats. - Information by UniProt
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Database links
- Entrez Gene: 252850 Zebrafish
- SwissProt: Q8JGW0 Zebrafish
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Alternative names
- Thbs4 antibody
- thrombospondin 4 antibody
- thrombospondin 4b antibody
see all
Images
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Anti-THBS4 antibody - N-terminal (ab211143) at 1/1000 dilution + Zebrafish muscle whole cell lysate at 30 µg
Predicted band size: 104 kDa7.5% SDS-PAGE
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Wholemount immunohistochemical analysis of 2 days-post-fertilization zebrafish embryo labeling THBS4 with ab211143 at 1/100 dilution.
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Wholemount immunohistochemical analysis of 2 days-post-fertilization zebrafish embryo labeling THBS4 with ab211143 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab211143 has been referenced in 3 publications.
- McAdow J et al. A pathogenic mechanism associated with myopathies and structural birth defects involves TPM2-directed myogenesis. JCI Insight 7:N/A (2022). PubMed: 35579956
- Unlu G et al. Phenome-based approach identifies RIC1-linked Mendelian syndrome through zebrafish models, biobank associations and clinical studies. Nat Med 26:98-109 (2020). PubMed: 31932796
- Kague E et al. Scleraxis genes are required for normal musculoskeletal development and for rib growth and mineralization in zebrafish. FASEB J 33:9116-9130 (2019). PubMed: 31100023