Overview

  • Product name
    Anti-Themis antibody [EPR7353]
    See all Themis primary antibodies
  • Description
    Rabbit monoclonal [EPR7353] to Themis
  • Tested applications
    Suitable for: WB, IP, ICC, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Themis (Q8N1K5).

  • Positive control
    • Jurkat and Human thymus lysates; Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
IP 1/10 - 1/100.
ICC 1/50 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
    • Sequence similarities
      Belongs to the themis family.
    • Post-translational
      modifications
      Phosphorylated on Tyr residues quickly after TCR stimulation.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C6orf190 antibody
      • C6orf207 antibody
      • MGC163388 antibody
      • Protein THEMIS antibody
      • SIGNALING PHOSPHOPROTEIN SPECIFIC FOR T CELLS antibody
      • SPOT antibody
      • THEMIS antibody
      • THMS1_HUMAN antibody
      • Thymocyte expressed molecule involved in selection antibody
      • Thymocyte selection associated antibody
      • Thymocyte selection pathway associated antibody
      • Thymocyte-expressed molecule involved in selection antibody
      • TSEPA antibody
      see all

    Images

    • All lanes : Anti-Themis antibody [EPR7353] (ab126771) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Human thymus lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 73 kDa
    • ab126771 at 1/10 dilution staining Themis in permeabilized Jurkat cells by Flow cytometry (red) compared with a rabbit IgG negative control (green).

    References

    This product has been referenced in:
    • Kinosada H  et al. HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors. PLoS Pathog 13:e1006120 (2017). IF ; Mouse . Read more (PubMed: 28046066) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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