Recombinant Anti-Themis antibody [EPR7354] (ab129174)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7354] to Themis
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Themis antibody [EPR7354]
See all Themis primary antibodies -
Description
Rabbit monoclonal [EPR7354] to Themis -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Themis aa 600-700. The exact sequence is proprietary.
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Positive control
- Human thymus and Jurkat lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7354 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa). |
Target
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Function
Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. -
Sequence similarities
Belongs to the themis family. -
Post-translational
modificationsPhosphorylated on Tyr residues quickly after TCR stimulation. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 387357 Human
- Omim: 613607 Human
- SwissProt: Q8N1K5 Human
- Unigene: 661756 Human
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Alternative names
- C6orf190 antibody
- C6orf207 antibody
- MGC163388 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab129174 has been referenced in 1 publication.
- Davies JL et al. Increased THEMIS First Exon Usage in CD4+ T-Cells Is Associated with a Genotype that Is Protective against Multiple Sclerosis. PLoS One 11:e0158327 (2016). WB ; Human . PubMed: 27438997