Thiol Quantification Assay Kit (Fluorometric) (ab112158) provides an ultrasensitive fluorometric assay to quantitate thiol content that exists in small molecules such as free cysteine, glutathione, and cysteine residues. The assay is based on a proprietary non-fluorescent dye tha generates a strongly fluorescent adduct upon reacting with a thiol compound. The signal produced by the adduct can ebe asily read by a fluorescence microplate reader at Ex/Em = 490/520 nm. In addition, both absorption and emission spectra of the thiol adduct are pH-independent, making the assay highly robust. There are few reagents or assay kits available for quantitating thiol content in biological systems, and the majority of commercial kits either lack sensitivity or have tedious protocols. The assay can detect as little as 1 picomole of cysteine or GSH in a 100 µL assay volume (10 nM).
Please note it is not possible to do a quantification in comparison to the standard.
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This product detects thiol groups on small molecules. If you want to detect thiol groups in proteins, we recommend Total Protein Thiol Quantification Assay Kit (Colorimetric) (ab219272).
|Assay Buffer||1 x 25ml|
|DMSO||1 x 200µl|
|GSH Standard||1 x 62µg|
|Thiol Green Indicator||1 vial|
Our Abpromise guarantee covers the use of ab112158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
GSH measured in cell lysates showing quantity (nmol) per 1 mln cells.
Samples with the concentration of 1e8 cells/mL were used. Samples were diluted 100-1000 fold.
GSH measured in biological fluids. Samples were diluted 10-1000 fold.
ab112158 has not yet been referenced specifically in any publications.