Anti-Thiophosphate ester antibody [51-8] (ab92570)


  • Product nameAnti-Thiophosphate ester antibody [51-8]
    See all Thiophosphate ester primary antibodies
  • Description
    Rabbit monoclonal [51-8] to Thiophosphate ester
  • SpecificityThis antibody is specific to thiophosphorylated amino acids.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    not applicable
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Thiophosphate ester. The exact sequence is proprietary.

  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Kevan Shokat at University of California at San Francisco.

    It is possible to use ab92570 with ab138911 as a kinase reaction reagent for the identification of direct kinase substrates as mentioned in Allen JJ et al. Nat Methods 4:511-6 (2007).

    After the kinase of interest has accepted ATP-γ-S (ab138911), p-Nitrobenzyl mesylate (ab138910) can be used to alkylate the thiophospholyation site on the substrates. ab92570 is introduced to identify the tagged substrates.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • Primary antibody notesab92570 can be used to detect kinase substrates containing thiophosphate esters following the kinase reaction as described by J. Allen and colleagues in Nature methods 2007. This antibody as allow researchers to follow this approach to study kinase-substrate relationships of interest. The antibody is also context independent in that it can recognize serine, threonine, and tyrosine thiophosphorylated residues in a multitude of kinase phosphorylation consensus motifs.
    In order for this antibody to work in WB and IP, the customer needs to perform a specific kinase reaction using ATP gamma S and PNBM.
    See specific Protocol at the bottom of this page for details.
  • ClonalityMonoclonal
  • Clone number51-8
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab92570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000.


  • RelevanceThiophosphate ester is used as fungicide.

Anti-Thiophosphate ester antibody [51-8] images

  • All lanes : Anti-Thiophosphate ester antibody [51-8] (ab92570) at 1/5000 dilution

    Lane 1 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug)
    Lane 2 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using alkylation with PNBM (2.5mM).
    Lane 3 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using ATP-gamma-S
    Lane 4 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using ATP-gamma-S followed by alkylation with PNBM (2.5mM).


References for Anti-Thiophosphate ester antibody [51-8] (ab92570)

This product has been referenced in:
  • Leissing F  et al. Substrate thiophosphorylation by Arabidopsis mitogen-activated protein kinases. BMC Plant Biol 16:48 (2016). Read more (PubMed: 26912131) »
  • Li YH  et al. AMP-Activated Protein Kinase Directly Phosphorylates and Destabilizes Hedgehog Pathway Transcription Factor GLI1 in Medulloblastoma. Cell Rep 12:599-609 (2015). Read more (PubMed: 26190112) »

See all 10 Publications for this product

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