Thioredoxin / TRX overexpression 293T lysate (whole cell) (ab94039)

Overview

  • Product nameThioredoxin / TRX overexpression 293T lysate (whole cell)
  • General notesab94039 is a 293T cell transfected lysate in which Human Thioredoxin / TRX 293T has been transiently over-expressed using a pCMV-Thioredoxin / TRX 293T plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55). Similarity: Belongs to the thioredoxin family. Contains 1 thioredoxin domain. PTM: In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins. In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Thioredoxin / TRX overexpression 293T lysate (whole cell) images

  • ab94039 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-Thioredoxin / TRX antibody (ab57675) at 1/500 dilution

    Lane 1 : Thioredoxin / TRX 293T Transfected Lysate - Positive Control (ab94039)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Thioredoxin / TRX overexpression 293T lysate (whole cell) (ab94039)

ab94039 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Questions for ab94039.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"