Anti-Thioredoxin 2 antibody [4C5] (ab16856)


  • Product nameAnti-Thioredoxin 2 antibody [4C5]
    See all Thioredoxin 2 primary antibodies
  • Description
    Mouse monoclonal [4C5] to Thioredoxin 2
  • Tested applicationsSuitable for: ELISA, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • 293T cell lysate



Our Abpromise guarantee covers the use of ab16856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
IP Use a concentration of 2 µg/ml.
  • Application notesIs unsuitable for WB.
  • Target

    • RelevanceThioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide isomerase(PDI) and a number of transcription factors such as p53, NF-kB and AP-1. Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.
    • Cellular localizationMitochondrial
    • Database links
    • Alternative names
      • mitochondrial thioredoxin antibody
      • MT-TRX antibody
      • MTRX antibody
      • thioredoxin 2 precursor antibody
      • Thioredoxin mitochondrial antibody
      • Thioredoxin2 antibody
      • TRX 2 antibody
      • TRX2 antibody
      • TXN 2 antibody
      • TXN2 antibody
      see all

    Anti-Thioredoxin 2 antibody [4C5] images

    • Immunoprecipitation analysis of 293T cell lysates using ab16856 at 2ug/ml of cell lysate.
      Western blot using another Thioredoxin 2 antibody (ab16836).

      Lane 1- Input
      Lane 2- Precipitates

    References for Anti-Thioredoxin 2 antibody [4C5] (ab16856)

    ab16856 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your call today. I've substituted ab71262 for ab16856 on the order, and this should ship out tonight for delivery tomorrow.

    Expiration date: August 11, 2012

    This code will give you 1 free primar...

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    Thank you for your phone call. I contacted the originator of ab16856 and they provided me with the following information. They did test ab16856 in Western blotting but found that it did not work. So, ab16856 works in IP and ELISA, but not Western blott...

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    Thank you for your telephone enquiry and also your gentle and kind approach. We do like working with co-operative customers and the majority of them highly appreciate our effort and time and are satisfied with our customer service. Here I am forwar...

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    Thank you for calling us regarding ab16856. I enclose the RIPA buffer that we recommend for mitochondrial extraction: RIPA buffer (RadioImmunoPrecipitation Assay) buffer: RIPA buffer contains the ionic detergent sodium deoxycholate as an activ...

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