Anti-Thioredoxin 2 antibody [71G4] (ab16857)

Overview

  • Product nameAnti-Thioredoxin 2 antibody [71G4]
    See all Thioredoxin 2 primary antibodies
  • Description
    Mouse monoclonal [71G4] to Thioredoxin 2
  • Tested applicationsSuitable for: ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab16857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IP Use a concentration of 2 µg/ml.

Target

  • RelevanceThioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide isomerase(PDI) and a number of transcription factors such as p53, NF-kB and AP-1. Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.
  • Cellular localizationMitochondrial
  • Database links
  • Alternative names
    • mitochondrial thioredoxin antibody
    • MT-TRX antibody
    • MTRX antibody
    • thioredoxin 2 precursor antibody
    • Thioredoxin mitochondrial antibody
    • Thioredoxin2 antibody
    • TRX 2 antibody
    • TRX2 antibody
    • TXN 2 antibody
    • TXN2 antibody
    see all

Anti-Thioredoxin 2 antibody [71G4] images

  • Immunoprecipitation analysis of 293T cell lysates using ab16857 at 2µg/ml lysate.
    Western blotting using another Thioredoxin 2 antibody.
    Lane 1- Input
    Lane 2- Precipitates

References for Anti-Thioredoxin 2 antibody [71G4] (ab16857)

ab16857 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 75 µg
Gel Running Conditions Reduced Denaturing (12.5)
Sample Rat Cell lysate - other (gonad)
Specification gonad
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Sep 17 2013

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in Western blotting. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"