Anti-Thioredoxin / TRX antibody [2B1] (ab1754)

Overview

  • Product nameAnti-Thioredoxin / TRX antibody [2B1]
    See all Thioredoxin / TRX primary antibodies
  • Description
    Mouse monoclonal [2B1] to Thioredoxin / TRX
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • Human placental tissue
  • General notesShould this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab1754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 10 - 50 µg/ml. Detects a band of approximately 12 kDa.
ELISA Use at an assay dependent concentration.
IHC-Fr 1/50. PubMed: 8577662

Target

  • FunctionParticipates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
    ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
  • Sequence similaritiesBelongs to the thioredoxin family.
    Contains 1 thioredoxin domain.
  • Post-translational
    modifications
    In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
    In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
  • Cellular localizationNucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADF antibody
    • ATL derived factor antibody
    • ATL-derived factor antibody
    • DKFZp686B1993 antibody
    • MGC61975 antibody
    • SASP antibody
    • Surface associated sulphydryl protein antibody
    • Surface-associated sulphydryl protein antibody
    • testicular tissue protein Li 199 antibody
    • THIO_HUMAN antibody
    • Thioredoxin antibody
    • thioredoxin delta 3 antibody
    • TRDX antibody
    • TRX 1 antibody
    • Trx antibody
    • TRX1 antibody
    • TXN antibody
    • TXN delta 3 antibody
    • TXN protein antibody
    • zgc:92903 antibody
    see all

References for Anti-Thioredoxin / TRX antibody [2B1] (ab1754)

This product has been referenced in:
  • Maddens B  et al. Chitinase-like proteins are candidate biomarkers for sepsis-induced acute kidney injury. Mol Cell Proteomics : (2012). WB ; Mouse . Read more (PubMed: 22233884) »
  • Ebrahimian T  et al. Differential regulation of thioredoxin and NAD(P)H oxidase by angiotensin II in male and female mice. J Hypertens 25:1263-71 (2007). Mouse . Read more (PubMed: 17563540) »

See all 3 Publications for this product

Product Wall

Thank you foryour reply.

I have not been able to link any of our antibodies to the one used in this article, did they suggest a catalogue number?

We have several antibodies against thioredoxin which may be of interest to you. The on...

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I am very pleased to hear you would like to accept our offer and test ab1754 in IP. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration da...

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Merci de votre intérêt pour nos anticorps. La TRX80 correspond effectivement aux 80 acides aminés N terminaux de TRX. J'ai donc cherché dans nos anticorps si nous avons un qui reconnait un épitope dans les premiers 80 acides aminés. Pou...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Ewing's cell lines TTC-466 and A673)
Loading amount 50 µg
Specification Ewing's cell lines TTC-466 and A673
Gel Running Conditions Reduced Denaturing (12)
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Dec 04 2008

Thank you for your enquiry. We make the following recommendations for use as positive controls; Superoxide dismutase 2 antibody (ab13533): Rat brain tissue extract. Superoxide dismutase 1 antibody (ab21968): Human brain tissue Thioredoxi...

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Thank you for your enquiry. The Abs that you have expressed an interest in have not been tested in conjunction with each other to use in a sandwich ELISA. These products are suitable for use either in direct or indirect ELISA. If you wish to set up a s...

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From the information we have the antibody was raised against a recombinant TRX grown in E.Coli. It is know to react with native TRX and the recombinant TRX used as the immunogen. Whether it will react with recombinant TRX in other E.Coli system we d...

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Thank you for your enquiry. We would like to mention that this product has been tested with human samples and as the datasheet indicates human recombinant full length protein Thioredoxin was used to raise this antibody. The cross-reactivity of a...

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Thank you for your enquiry. All the information that we have on this antibody is located on the online datasheet. We do not have a specific ELISA protocol for this antibody. Compatible secondaries for ab1754 are located at the bottom of the datasheet, ...

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I have been unsuccessful in locating information to suggest that the epitope recognised by this antibody has been characterised. Sorry we cannot provide you with any further information.

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