• Product nameAnti-Thioredoxin / TRX antibody [8A1]
    See all Thioredoxin / TRX primary antibodies
  • Description
    Mouse monoclonal [8A1] to Thioredoxin / TRX
  • Tested applicationsSuitable for: ELISA, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • 293T whole cell lysate.



Our Abpromise guarantee covers the use of ab16845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
IP Use a concentration of 1 - 2 µg/ml.
WB Use at an assay dependent dilution.


  • FunctionParticipates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
    ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
  • Sequence similaritiesBelongs to the thioredoxin family.
    Contains 1 thioredoxin domain.
  • Post-translational
    In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
    In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
  • Cellular localizationNucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADF antibody
    • ATL derived factor antibody
    • ATL-derived factor antibody
    • DKFZp686B1993 antibody
    • MGC61975 antibody
    • SASP antibody
    • Surface associated sulphydryl protein antibody
    • Surface-associated sulphydryl protein antibody
    • testicular tissue protein Li 199 antibody
    • THIO_HUMAN antibody
    • Thioredoxin antibody
    • thioredoxin delta 3 antibody
    • TRDX antibody
    • TRX 1 antibody
    • Trx antibody
    • TRX1 antibody
    • TXN antibody
    • TXN delta 3 antibody
    • TXN protein antibody
    • zgc:92903 antibody
    see all

Anti-Thioredoxin / TRX antibody [8A1] images

  • Immunoprecipitation analysis of 293T cell lysates using ab16845.

    Lane 1: Input.

    Lane 2: Precipitates.

References for Anti-Thioredoxin / TRX antibody [8A1] (ab16845)

This product has been referenced in:
  • Yu Y & Richardson DR Cellular iron depletion stimulates the JNK and p38 MAPK signaling transduction pathways, dissociation of ASK1-thioredoxin, and activation of ASK1. J Biol Chem 286:15413-27 (2011). IP . Read more (PubMed: 21378396) »
  • Tipple TE  et al. Alterations of the thioredoxin system by hyperoxia: implications for alveolar development. Am J Respir Cell Mol Biol 41:612-9 (2009). Read more (PubMed: 19244202) »

See all 2 Publications for this product

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Thank you for your enquiry. The Abs that you have expressed an interest in have not been tested in conjunction with each other to use in a sandwich ELISA. These products are suitable for use either in direct or indirect ELISA. If you wish to set up a s...

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