• Product nameAnti-Thioredoxin / TRX antibody
    See all Thioredoxin / TRX primary antibodies
  • Description
    Rabbit polyclonal to Thioredoxin / TRX
  • Tested applicationsSuitable for: IP, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Cat, Human
    Predicted to work with: Rat, Sheep, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Thioredoxin/ TRX.

    (Peptide available as ab27815.)

  • Positive control
    • HeLa, Jurkat and A431 whole cell lysates.



Our Abpromise guarantee covers the use of ab26320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
ICC/IF 1/200 - 1/1000.
IHC-P 1/2000.


  • FunctionParticipates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
    ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
  • Sequence similaritiesBelongs to the thioredoxin family.
    Contains 1 thioredoxin domain.
  • Post-translational
    In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
    In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
  • Cellular localizationNucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADF antibody
    • ATL derived factor antibody
    • ATL-derived factor antibody
    • DKFZp686B1993 antibody
    • MGC61975 antibody
    • SASP antibody
    • Surface associated sulphydryl protein antibody
    • Surface-associated sulphydryl protein antibody
    • THIO_HUMAN antibody
    • Thioredoxin antibody
    • TRDX antibody
    • TRX 1 antibody
    • Trx antibody
    • TRX1 antibody
    • TXN antibody
    • TXN protein antibody
    • zgc:92903 antibody
    see all

Anti-Thioredoxin / TRX antibody images

  • Thioredoxin / TRX was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Thioredoxin / TRX and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab26320.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 12kDa; Thioredoxin / TRX

  • ab26320, at 1/200 dilution, staining human TRX in assynchronous HeLa cells by ICC/IF.

    See Abreview

  • Immunofluorescent detection of Thioredoxin /TRX using antibody ab26320. Chinese Hamster Cell (Ovary cells) were fixed in formaldehyde and permeabilized in 0.1% Triton X in PBS/3% BSA. Primary antibody was incubated at 1/1000 for 1h @ 4°C in PBS containing 3% BSA.  Secondary antibody: anti-rabbit conjugated to Alexa Fluor® 568 used @ 1/300.  CHO cells co-transfected with plasmids encoding GFP and a mutated form of human thioredoxin which does not enter the nucleus, and labelled using ab26320 (1/1000) and Alexa 568 (1/300). Panel [A] demonstrates GFP immunoreactivity. [B ]thioredoxin immunoreactity; many of the same cells are immunoreactive for both GFP and thioredoxin. Panel [D] thioredoxin primary antibody was omitted, hence there is no immunoeactivity.

    See Abreview

  • ab26320 staining Thioredoxin/TRX in Human lung tissue sections by IHC-P (Formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5 minutes of peroxidase block followed by 10 minutes of protein block at 20°C; antigen retrieval was heat mediated in retrieval solution. Samples were incubated with primary antibody (1/2000 in antibody diluent) for 45 minutes at 20°C. An undiluted HRP-conjugated polymer goat anti-mouse/rabbit IgG polyclonal polymer was used as the secondary antibody.

    See Abreview

  • Anti-Thioredoxin / TRX antibody (ab26320) at 1 µg/ml + Recombinant human Thioredoxin / TRX protein (ab51064) at 0.01 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 12 kDa

    Exposure time : 30 seconds
  • Anti-Thioredoxin / TRX antibody (ab26320) at 1 µg/ml + HeLa whole cell lysate at 20 µg

    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 12 kDa
    Observed band size : 12 kDa

    ab26320 detects a band of 12 kDa in HeLa, Jurkat and A431 whole cell lysates. The data for the Jurkat and A431 lysates is not shown.


References for Anti-Thioredoxin / TRX antibody (ab26320)

This product has been referenced in:
  • Shearn CT  et al. Increased carbonylation of the lipid phosphatase PTEN contributes to Akt2 activation in a murine model of early alcohol-induced steatosis. Free Radic Biol Med 65:680-92 (2013). Mouse . Read more (PubMed: 23872024) »
  • Jensen PL  et al. Proteomic Analysis of Human Blastocoel Fluid and Blastocyst Cells. Stem Cells Dev : (2012). WB, IHC-P ; Human . Read more (PubMed: 23148560) »

See all 6 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Hep G2)
Specification Hep G2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Dec 26 2014

Without reduced and denatured samples, since the antibody was raised against a peptide it may not be able to recognize its epitope under the non-reduced, non-denatured conditions of the redox WB.

Since you're samples in the redox WB are non-re...

Read More

Thank you foryour reply.

I have not been able to link any of our antibodies to the one used in this article, did they suggest a catalogue number?

We have several antibodies against thioredoxin which may be of interest to you. The on...

Read More

Merci de votre intérêt pour nos anticorps. La TRX80 correspond effectivement aux 80 acides aminés N terminaux de TRX. J'ai donc cherché dans nos anticorps si nous avons un qui reconnait un épitope dans les premiers 80 acides aminés. Pou...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Mar 22 2010

Application Immunocytochemistry/ Immunofluorescence
Sample Chinese Hamster Cell (Ovary cells)
Specification Ovary cells
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X in PBS/3% BSA

Mr. Carl Hobbs

Verified customer

Submitted Feb 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde

Dr. Kirk McManus

Verified customer

Submitted Dec 18 2006