Anti-THOC5 antibody [EPR6905(B)] (ab124996)

Overview

  • Product nameAnti-THOC5 antibody [EPR6905(B)]
    See all THOC5 primary antibodies
  • Description
    Rabbit monoclonal [EPR6905(B)] to THOC5
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human THOC5.

  • Positive control
    • 293T, HeLa, NIH 3T3 and A549 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD) KD = 4.40 x 10 -11 M Learn more about KD
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR6905(B)
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionComponent the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction beteen KSHV ORF57 protein and ALYREF/THOC4. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA. Regulates the expression of myeloid transcription factors CEBPA, CEBPB and GAB2 by enhancing the levels of phosphatidylinositol 3,4,5-trisphosphate. May be involved in the differentiation of granulocytes and adipocytes. Essential for hematopoietic primitive cell survival and plays an integral role in monocytic development.
    • Tissue specificityUbiquitously expressed.
    • Sequence similaritiesBelongs to the THOC5 family.
    • Post-translational
      modifications
      Phosphorylated on tyrosine upon binding to activated CSF1R; which causes a dissociation of the two proteins. Phosphorylation on Ser-5 and/or Ser-6 is required for nuclear export. Phosphorylated on Thr-328 in insulin-stimulated adipocytes (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus. Cytoplasm. Shuttles between nucleus and cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • C22orf19 antibody
      • Chromosome 22 open reading frame 19 antibody
      • Fmip antibody
      • Fms interacting protein antibody
      • fSAP79 antibody
      • Functional spliceosome-associated protein 79 antibody
      • hTREX90 antibody
      • KIAA0983 antibody
      • LOC400922 antibody
      • NF2/meningioma region protein pK1.3 antibody
      • PK1.3 antibody
      • Placental protein 39.2 antibody
      • PP39.2 antibody
      • THO complex 5 antibody
      • THO complex subunit 5 antibody
      • THO complex subunit 5 homolog antibody
      • thoc5 antibody
      • THOC5_HUMAN antibody
      see all

    Anti-THOC5 antibody [EPR6905(B)] images

    • All lanes : Anti-THOC5 antibody [EPR6905(B)] (ab124996) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : NIH 3T3 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 79 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-THOC5 antibody [EPR6905(B)] (ab124996)

    ab124996 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"