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Overview
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Product nameAnti-THOC7 antibody
See all THOC7 primary antibodies -
DescriptionRabbit polyclonal to THOC7
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Chicken, Cow, Xenopus tropicalis
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Immunogen
Recombinant protein fragment, corresponding to a region within amino acids 6-193 of Human THOC7 (UniProt ID: Q6I9Y2).
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Positive control
- 293T, A431 and Jurkat cell lysates; mouse brain lysate; A431 cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 24 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionComponent of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4.
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Sequence similaritiesBelongs to the THOC7 family.
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Cellular localizationCytoplasm. Nucleus. Interaction with THOC5 is required for nuclear localization.
- Information by UniProt
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Database links
- Entrez Gene: 416077 Chicken
- Entrez Gene: 510250 Cow
- Entrez Gene: 80145 Human
- Entrez Gene: 66231 Mouse
- Entrez Gene: 394707 Xenopus tropicalis
- Omim: 611965 Human
- SwissProt: Q3SZ60 Cow
- SwissProt: Q6I9Y2 Human
see all -
Alternative names
- FLJ23445 antibody
- fSAP24 antibody
- Functional spliceosome associated protein 24 antibody
see all
Images
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Western blot - Anti-THOC7 antibody (ab155218)All lanes : Anti-THOC7 antibody (ab155218) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 24 kDa
12% SDS PAGE -
Western blot - Anti-THOC7 antibody (ab155218)Anti-THOC7 antibody (ab155218) at 1/1000 dilution + mouse brain lysate at 50 µg
Predicted band size: 24 kDa
15% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-THOC7 antibody (ab155218)Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, labeling THOC7 using ab155218 at a 1/200 dilution. The image in the lower panel was costained with Hoechst 33342.
References
ab155218 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"