Overview

  • Product name
  • Description
    Rabbit polyclonal to THOC7
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Cow, Xenopus tropicalis
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 6-193 of Human THOC7 (UniProt ID: Q6I9Y2).

  • Positive control
    • 293T, A431 and Jurkat cell lysates; mouse brain lysate; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab155218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4.
  • Sequence similarities
    Belongs to the THOC7 family.
  • Cellular localization
    Cytoplasm. Nucleus. Interaction with THOC5 is required for nuclear localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ23445 antibody
    • fSAP24 antibody
    • Functional spliceosome associated protein 24 antibody
    • Functional spliceosome-associated protein 24 antibody
    • hTREX30 antibody
    • Ngg1 interacting factor 3 like 1 binding protein 1 antibody
    • Ngg1 interacting factor 3 like protein 1 binding protein 1 antibody
    • Ngg1-interacting factor 3-like protein 1-binding protein 1 antibody
    • NIF3L1 binding protein 1 antibody
    • NIF3L1-binding protein 1 antibody
    • NIF3L1BP1 antibody
    • THO complex 7 homolog (Drosophila) antibody
    • THO complex 7 homolog antibody
    • THO complex subunit 7 homolog antibody
    • THOC 7 antibody
    • thoc7 antibody
    • THOC7_HUMAN antibody
    see all

Images

  • All lanes : Anti-THOC7 antibody (ab155218) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 24 kDa
  • Anti-THOC7 antibody (ab155218) at 1/1000 dilution + mouse brain lysate at 50 µg

    Predicted band size : 24 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, labeling THOC7 using ab155218 at a 1/200 dilution. The image in the lower panel was costained with Hoechst 33342.

References

ab155218 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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