Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted (ab17785)

Overview

  • Product nameAnti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted
    See all Thomsen-Friedenreich Antigen primary antibodies
  • Description
    Mouse monoclonal [SPM320] to Thomsen-Friedenreich Antigen, prediluted
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Tissue/ cell preparation (Human). Neuraminidase-treated human red blood cells.

  • EpitopeGalß1-3GalNAc
  • Positive control
    • KG1 cells. Human colorectal carcinoma tissues.

Properties

  • FormPrediluted
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.6
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 1% BSA
  • Concentration information loading...
  • PurityPurified IgM
  • ClonalityMonoclonal
  • Clone numberSPM320
  • IsotypeIgM
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceThe Thomsen Friedenreich antigen acts as an oncofoetal antigen, with low expression in normal adult tissues but increasing to foetal levels of expression in hyperplasia or malignancy. It is considered as a pan carcinoma marker. Can be applied for the detection of cells with TF antigen and is especially applicable for sensitive determination of neuraminidases.
  • Cellular localizationCell Membrane
  • Alternative names
    • CD176 antibody
    • TF antibody
    • TF antigen antibody

Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted images

  • Human colon adenocarcinoma stained with ab17785 (prediluted)

References for Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted (ab17785)

This product has been referenced in:
  • Bhattacharyya S  et al. Amelioration of aspirin induced oxidative impairment and apoptotic cell death by a novel antioxidant protein molecule isolated from the herb Phyllanthus niruri. PLoS One 9:e89026 (2014). Mouse . Read more (PubMed: 24586486) »
  • Kumagai H  et al. Multifunctional nanobeacon for imaging Thomsen-Friedenreich antigen-associated colorectal cancer. Int J Cancer : (2012). Human . Read more (PubMed: 23055136) »

See all 2 Publications for this product

Product Wall

ab17785 is supplied in a TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"