Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted (ab17785)

Overview

  • Product name
    Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted
  • Description
    Mouse monoclonal [SPM320] to Thomsen-Friedenreich Antigen, prediluted
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Tissue/ cell preparation (Human). Neuraminidase-treated human red blood cells.

  • Epitope
    Galß1-3GalNAc
  • Positive control
    • KG1 cells. Human colorectal carcinoma tissues.

Properties

  • Form
    Prediluted
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.6
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 1% BSA
  • Concentration information loading...
  • Purity
    Purified IgM
  • Clonality
    Monoclonal
  • Clone number
    SPM320
  • Isotype
    IgM
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    The Thomsen Friedenreich antigen acts as an oncofoetal antigen, with low expression in normal adult tissues but increasing to foetal levels of expression in hyperplasia or malignancy. It is considered as a pan carcinoma marker. Can be applied for the detection of cells with TF antigen and is especially applicable for sensitive determination of neuraminidases.
  • Cellular localization
    Cell Membrane
  • Alternative names
    • CD176 antibody
    • TF antibody
    • TF antigen antibody

Images

  • Human colon adenocarcinoma stained with ab17785 (prediluted)

References

This product has been referenced in:
  • Bhattacharyya S  et al. Amelioration of aspirin induced oxidative impairment and apoptotic cell death by a novel antioxidant protein molecule isolated from the herb Phyllanthus niruri. PLoS One 9:e89026 (2014). Mouse . Read more (PubMed: 24586486) »
  • Kumagai H  et al. Multifunctional nanobeacon for imaging Thomsen-Friedenreich antigen-associated colorectal cancer. Int J Cancer : (2012). Human . Read more (PubMed: 23055136) »

See all 2 Publications for this product

Customer reviews and Q&As

ab17785 is supplied in a TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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