THP1 nuclear extract lysate (PMA stimulated) (ab14901)

Overview

  • Product nameTHP1 nuclear extract lysate (PMA stimulated)
    See all THP1 lysates
  • Description
    THP1 nuclear extract lysate
  • General notesCell line: THP1 (Human acute monocytic leukemia).
    To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.
    The protein content has been determined by a Bradford-based assay.
    The THP-1 nuclear extracts have been collected in Lysis Buffer after a 24-hour incubation with or without PMA (10 ng/ml).

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.50
    Constituents: 0.48% HEPES, 2.34% Sodium chloride, 20% Glycerol, 0.003% EDTA, 0.042% Sodium fluoride, 0.0002% Sodium molybdate, 0.018% Sodium orthovanadate, 0.216% Beta glycerophosphate, 0.015% DTT
    Note: With Protease inhibitors.
  • Concentration information loading...
  • Research areas
  • BackgroundProperties: differentiation; lysozyme synthesis; phagocytosis; Fc receptor; IL-1 prod.; C3b receptor. The cell line can differentiate into macrophage-like cells (phagocytic) and can be used for induction of differentiation studies. When stimulated with PMA cells have been reported to differentialte into a monocytic pathway and release arachidonic acid and prostanoids.

THP1 nuclear extract lysate (PMA stimulated) images

  • Each lot has been tested for PPAR-gamma activation by using TransAM™ PPAR-gamma Kits. The signal intensity for PPAR-gamma activation in each lot is compared to the signal intensity obtained with extracts from unstimulated THP-1 cells (see figure). After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of PPAR-gamma activation in a given cell type.

References for THP1 nuclear extract lysate (PMA stimulated) (ab14901)

ab14901 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. For the stimulation of THP1 cells with PMA please perform the following; Grow THP-1 cells at a density range of 5-8 x 10exp5 c/ml., add PMA to a final concentration of 10 ng/ml to stimulate the cells. Do not add anyt...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"