The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionTranscription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes.
ab111211, at 3.75 µg/ml, staining ThPok in formalin fixed, paraffin embedded Human adrenal tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase streptavidin and chromogen.
ab111211, at 3.75 µg/ml, staining ThPok in formalin fixed, paraffin embedded Human testis tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase streptavidin and chromogen.
References for Anti-ThPok antibody (ab111211)
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