Anti-ThPok antibody (ab23388)

Overview

  • Product nameAnti-ThPok antibody
    See all ThPok primary antibodies
  • Description
    Rabbit polyclonal to ThPok
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide, derived from a region between residues 291-345 of Human ThPok.

  • Positive control
    • HepG2 cell lysate, Human heart and human lung tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab23388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionTranscription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes.
  • Sequence similaritiesContains 1 BTB (POZ) domain.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • c Krox antibody
    • cKrox antibody
    • DKFZp686G01254 antibody
    • hc Krox antibody
    • hcKrox antibody
    • Krueppel-related zinc finger protein cKrox antibody
    • Kruppel related zinc finger protein c Krox antibody
    • Kruppel related zinc finger protein cKrox antibody
    • Kruppel related zinc finger protein hcKrox antibody
    • T helper inducing POZ/Kruppel like factor antibody
    • T-helper-inducing POZ/Krueppel-like factor antibody
    • Th-POK antibody
    • ThPOK antibody
    • ZBT7B_HUMAN antibody
    • ZBTB 15 antibody
    • ZBTB 7B antibody
    • ZBTB15 antibody
    • ZBTB7B antibody
    • ZFP 67 antibody
    • Zfp-67 antibody
    • ZFP67 antibody
    • Zinc finger and BTB domain containing 15 antibody
    • Zinc finger and BTB domain containing 7B antibody
    • Zinc finger and BTB domain containing protein 15 antibody
    • Zinc finger and BTB domain containing protein 7B antibody
    • Zinc finger and BTB domain-containing protein 15 antibody
    • Zinc finger and BTB domain-containing protein 7B antibody
    • Zinc finger protein 67 homolog antibody
    • Zinc finger protein 857B antibody
    • Zinc finger protein Th POK antibody
    • Zinc finger protein Th-POK antibody
    • Zinc finger protein ThPOK antibody
    • ZNF857B antibody
    see all

Anti-ThPok antibody images



  • Predicted band size : 60 kDa
    Western blot analysis of Human fetal liver tissue lysate labeling ThPok with ab23388 at 1.0µg/ml.
  • Anti-ThPok antibody (ab23388) + HepG2 cell lysate (20ug)

    Secondary
    HRP conjugated anti-Rabbit IgG diluted in 1: 50,000 - 100,000.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    ab23388 titration 0.004ug/ml.
  • Immunohistochemical analysis of ThPok expression in paraffin embedded human heart tissue section. ab23388 was used at 4 ug/ml. Arrows indicate the positively labelled myocardial cells.

  • Immunohistochemical analysis of ThPok expression in paraffin embedded human lung tissue section. ab23388 was used at 4 ug/ml. Arrows indicate the positively labelled alveolar cells.

  • All lanes : Anti-ThPok antibody (ab23388) at 1.25 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 60 kDa

References for Anti-ThPok antibody (ab23388)

ab23388 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Flow Cytometry
Fixation eBioscience transcription factor fixation buffer
Permeabilization Yes - eBioscience transcription factor permeabilization buffer
Sample Human Cell (Thymocytes and PBMCs)
Specification Thymocytes and PBMCs
Gating Strategy Lymphocytes - doublet exclusion - CD4+ vs. CD8+
Preparation Cell harvesting/tissue preparation method: meshed/Ficoll
Sample buffer: PBS/BSA
Username

Abcam user community

Verified customer

Submitted Jun 23 2014

The better choice will be ab70991, as the immunogen is 100% conserved in rat ThPok. The immunogen for ab23388 is conserved 85%, so reactivity is predicted, but is less probable than ab70991 reactivity.

Thank you for sending this information.

If you would like, I can issue free of charge replacements of the antibody and lysate with another anti-ThPok antibody such as ab23388 or ab70991 and our recommended positive control lysate. Like ab9...

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Thank you for your enquiry.

I am sorry to confirm that non of our thPok antibodies have been tested and guaranteed in Xenopus tropicalis. I have checked the alignment of the sequence you kindly provided with the immunogen sequences for the a...

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Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. To our ...

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Thank you for your phone call. The sequence of the synthetic peptide used for immunization is as follows: AQGGGPPLSPEELGSDEDAIDPDLMAYLSSLHQDNLAPGLDSQDKLVRKRRSQMP. It represents a part of a region from AA residue 291 to AA residue 345 of ZFP67 (NP_05695...

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