Anti-Thrombin antibody [5G9] (ab17199)
Key features and details
- Mouse monoclonal [5G9] to Thrombin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Thrombin antibody [5G9]
See all Thrombin primary antibodies -
Description
Mouse monoclonal [5G9] to Thrombin -
Host species
Mouse -
Specificity
Binds to human thrombin and to a complex of human thrombin with the thrombin inhibitor hirudin. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Thrombin. Thrombin isolated from activated human plasma
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Epitope
not mapped -
Positive control
- purified alpha thrombin IHC-P: Human liver FFPE tissue sections.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
5G9 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 70 kDa).
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IHC-P | (2) |
Use a concentration of 10 µg/ml.
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Notes |
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WB
1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 70 kDa). |
IHC-P
Use a concentration of 10 µg/ml. |
Target
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Function
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Factor II deficiency
Ischemic stroke
Thrombophilia due to thrombin defect
Pregnancy loss, recurrent, 2 -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). -
Cellular localization
Secreted, extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 2147 Human
- Omim: 176930 Human
- SwissProt: P00734 Human
- Unigene: 655207 Human
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Alternative names
- Coagulation factor II antibody
- Coagulation factor II thrombin antibody
- EC 3.4.21.5 antibody
see all
Images
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ab17199 staining Thrombin in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated horse anti-mouse IgG polyclonal was used as the secondary antibody.
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IHC image of Thrombin staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17199, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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All lanes : Anti-Thrombin antibody [5G9] (ab17199) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human Plasma Total Protein Lysate
Lane 3 :Hep G2 nuclear extract lysate (ab14660)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Thrombin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab17199 has been referenced in 2 publications.
- Tang X et al. Transferrin plays a central role in coagulation balance by interacting with clotting factors. Cell Res 30:119-132 (2020). PubMed: 31811276
- Coppin L et al. Clinical Protocol to Prevent Thrombogenic Effect of Liver-Derived Mesenchymal Cells for Cell-Based Therapies. Cells 8:N/A (2019). PubMed: 31394759