Overview

  • Product nameThrombin (Factor II) Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.7%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.3 ng/ml
  • Range
    0.313 ng/ml - 20 ng/ml
  • Recovery

    98 %

  • Assay time
    4h 0m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Thrombin (Factor II) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombin concentrations in cell culture supernatants. This assay recognizes both natural and recombinant Human thrombin.

    A Thrombin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Thrombin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Thrombin captured in plate.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent dilution.

Thrombin (Factor II) Human ELISA Kit images

  • Representative Standard Curve Using ab108909.

Protocols

References for Thrombin (Factor II) Human ELISA Kit (ab108909)

This product has been referenced in:
  • Liu JF  et al. Thrombin induces heme oxygenase-1 expression in human synovial fibroblasts through protease-activated receptor signaling pathways. Arthritis Res Ther 14:R91 (2012). Sandwich ELISA ; Human . Read more (PubMed: 22541814) »

See 1 Publication for this product

Product Wall

Thank you for your patience while I looked into this.

The lab has sent us thereplacement vials of the diluent and wash buffer. We are sending these to you free of charge on order. You should receive these tomorrow.

I hope this infor...

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Thank you for contacting Abcam regarding ab108909.


Just to confirm what we discussed over the phone:


Thrombin Standard: Purified human Thrombin in a buffered protein base (40 ng, lyophilized).

Standard Curve: Recons...

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Thank you for contacting us.

I heard back from the lab and they recommend that youdispose the Wash Buffer and the Diluent if you mixed them together.

Wash Buffer is 20x and should be diluted with water (1 part Wash Buffer + 19 parts...

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Thank you for calling Abcam earlier today.
I have talked to the lab and they do not think there is protein the same size as thrombin in the buffer based, but we cannot be 100% sure. The exact components of the buffer are proprietary and so I am una...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"