Anti-PAR1/Thrombin Receptor antibody [5E2E6] (ab233741)
Key features and details
- Mouse monoclonal [5E2E6] to PAR1/Thrombin Receptor
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PAR1/Thrombin Receptor antibody [5E2E6]
See all PAR1/Thrombin Receptor primary antibodies -
Description
Mouse monoclonal [5E2E6] to PAR1/Thrombin Receptor -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PAR1/Thrombin Receptor aa 1-200. (Expressed in E.coli).
Database link: P25116 -
Positive control
- WB: Recombinant human PAR1/Thrombin Receptor protein; PAR1/Thrombin Receptor -hIgGFc transfected HEK293 cell lysate. IHC-P: Human rectum cancer.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
5E2E6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233741 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/1000.
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WB |
1/500 - 1/2000.
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Notes |
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IHC-P
1/200 - 1/1000. |
WB
1/500 - 1/2000. |
Target
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Function
High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development. -
Tissue specificity
Platelets and vascular endothelial cells. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsA proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2149 Human
- Omim: 187930 Human
- SwissProt: P25116 Human
- Unigene: 482562 Human
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Alternative names
- CF2R antibody
- Coagulation factor II (thrombin) receptor antibody
- Coagulation factor II receptor antibody
see all
Images
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Formalin-fixed, paraffin-embedded human rectum cancer tissue stained for PAR1/Thrombin Receptor using ab233741 at 1/200 dilution in immunohistochemical analysis. DAB staining.
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All lanes : Anti-PAR1/Thrombin Receptor antibody [5E2E6] (ab233741) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : PAR1/Thrombin Receptor-hIgGFc transfected HEK-293 cell lysate -
Anti-PAR1/Thrombin Receptor antibody [5E2E6] (ab233741) at 1/500 dilution + Recombinant human PAR1/Thrombin Receptor protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab233741 has been referenced in 1 publication.
- Giannella A et al. PAR-4/Ca2+-calpain pathway activation stimulates platelet-derived microparticles in hyperglycemic type 2 diabetes. Cardiovasc Diabetol 20:77 (2021). PubMed: 33812377