Overview

  • Product name
    Anti-Thrombin Receptor antibody
    See all Thrombin Receptor primary antibodies
  • Description
    Rabbit polyclonal to Thrombin Receptor
  • Specificity
    ab63445 detects endogenous levels of total Thrombin Receptor protein.
  • Tested applications
    Suitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Thrombin Receptor (N terminal).

  • Positive control
    • HeLa cell extracts treated with Nocodazole (1ug/ml, 18hours).

Properties

Applications

Our Abpromise guarantee covers the use of ab63445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • Function
    High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.
  • Tissue specificity
    Platelets and vascular endothelial cells.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
    Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CF2R antibody
    • Coagulation factor II (thrombin) receptor antibody
    • Coagulation factor II receptor antibody
    • F2R antibody
    • HTR antibody
    • PAR 1 antibody
    • PAR-1 antibody
    • PAR1 antibody
    • PAR1_HUMAN antibody
    • Protease activated receptor 1 antibody
    • Proteinase activated receptor 1 antibody
    • Proteinase-activated receptor 1 antibody
    • Thrombin receptor antibody
    • TR antibody
    see all

Images

  • All lanes : Anti-Thrombin Receptor antibody (ab63445) at 1/500 dilution

    Lane 1 : HeLa cell extracts treated with Nocodazole (1ug/ml, 18hours)
    Lane 2 : HeLa cell extracts treated with Nocodazole (1ug/ml, 18hours) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 21 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab63445 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63445, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab63445 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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