Anti-Thrombomodulin antibody [PBS-01] (ab6980)

Overview

  • Product nameAnti-Thrombomodulin antibody [PBS-01]
    See all Thrombomodulin primary antibodies
  • Description
    Mouse monoclonal [PBS-01] to Thrombomodulin
  • Tested applicationsSuitable for: ELISA, WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Rabbit, Human
    Does not react with: Pig
  • Immunogen

    Recombinant full length protein (Human).

  • EpitopeEGF domain 5 of thrombomodulin.

Properties

Applications

Our Abpromise guarantee covers the use of ab6980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionThrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
  • Tissue specificityEndothelial cells are unique in synthesizing thrombomodulin.
  • Involvement in diseaseDefects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
    Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similaritiesContains 1 C-type lectin domain.
    Contains 6 EGF-like domains.
  • Post-translational
    modifications
    N-glycosylated.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHUS 6 antibody
    • AHUS6 antibody
    • BDCA 3 antibody
    • BDCA3 antibody
    • CD 141 antibody
    • CD141 antibody
    • CD141 antigen antibody
    • Fetomodulin antibody
    • Thbd antibody
    • THPH12 antibody
    • THRM antibody
    • Thrombomodulin antibody
    • TM antibody
    • TRBM_HUMAN antibody
    see all

Anti-Thrombomodulin antibody [PBS-01] images

  • Ab6980 staining human normal lung endothelium. Staining is localized to the membrane.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Overlay histogram showing THP1 cells stained with ab6980 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6980, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Anti-Thrombomodulin antibody [PBS-01] (ab6980) at 1/100 dilution + whole cell lysate prepared from human aortic endothelial cells at 30 µg

    Secondary
    HRP conjugated anti-mouse IgG at 1/4000 dilution
    Developed using the ECL technique

    Observed band size : 95 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes

    Image courtesy of an anonymous Abreview.

    36kDa band is GAPDH in the same lane.

References for Anti-Thrombomodulin antibody [PBS-01] (ab6980)

This product has been referenced in:
  • Kim SD  et al. Thrombomodulin Expression in Tissues From Dogs With Systemic Inflammatory Disease. Vet Pathol 53:797-802 (2016). Read more (PubMed: 26926084) »
  • Yamaji-Kegan K  et al. Hypoxia-induced mitogenic factor (FIZZ1/RELMa) induces endothelial cell apoptosis and subsequent interleukin-4-dependent pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol 306:L1090-103 (2014). Read more (PubMed: 24793164) »

See all 7 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Baboon Tissue sections (Heart)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization No
Specification Heart
Blocking step Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative 10% NBF
Username

Ms. Premier Laboratory

Verified customer

Submitted Apr 26 2016

Application Immunohistochemistry (Frozen sections)
Sample Pig Tissue sections (Heart)
Permeabilization No
Specification Heart
Blocking step Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative Acetone
Username

Ms. Premier Laboratory

Verified customer

Submitted Apr 19 2016

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (heart and lung)
Permeabilization No
Specification heart and lung
Blocking step Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative Acetone
Username

Ms. Premier Laboratory

Verified customer

Submitted Apr 19 2016

Thank you for contacting us.

ab6980 binds within the EGF 5-6 domain of human TM, rather distant from the N-terminus of the protein.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more a...

Read More

Thank you for your patience as I have looked into your inquiry.


Unfortunately, I have not been able to obtain any additional information from the originating laboratory regarding references or additional data in which this antibody was us...

Read More

Thank you for your reply and apologies for the confusion when I asked for the original Abcam order information, I meant the order information for ab6980.

The second order number you provided was exactly what I needed and I have now sent you a...

Read More

Thank you for your reply.

As mentioned previously we do guarantee our products for 6 months from the date of delivery, I mention this as the order number you provided is from September 2012 and ab6980 was not on that order (also the customer ...

Read More

Thank you for your reply.

As mentioned previously, this antibody is covered under our Abpromise and so if we cannot help resolve these issues, then ill be happy to either replace or refund this antibody.

Based on the information you...

Read More

Thank you for contacting Abcam.

The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples . If we cannot resolve the issue you are having with the antibody then I would be happy to e...

Read More

Thank you for confirming the details of your protocol. It is really strange that you're seeing such variable results with this antibody. I would recommend trying to block with 5% BSA instead of milk to see if that decreases the high background ...

Read More

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"