Overview

  • Product nameThrombomodulin (CD141) Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Serum 6 3.9%
    Inter-assay
    Sample n Mean SD CV%
    B 8 3.2ng/ml 0.3 10.8%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.31 ng/ml
  • Range
    0.625 ng/ml - 20 ng/ml
  • Recovery

    109 %

  • Assay time
    1h 45m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Thrombomodulin (CD141) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombomodulin (CD141) in serum, plasma, buffered solutions and supernatants.

    A monoclonal antibody specific for Thrombomodulin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Thrombomodulin concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Thrombomodulin are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Thrombomodulin present in the samples.

    This kit will recognize both endogenous and recombinant Human Thrombomodulin.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-Thrombomodulin Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    Control Silver 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
    Thrombomodulin Microplate (12 x 8 well strips) 1 unit 2 units
    Thrombomodulin Standard (lyophilized) Yellow 2 vials 4 vials
  • Research Areas
  • FunctionThrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
  • Tissue specificityEndothelial cells are unique in synthesizing thrombomodulin.
  • Involvement in diseaseDefects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
    Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similaritiesContains 1 C-type lectin domain.
    Contains 6 EGF-like domains.
  • Post-translational
    modifications
    N-glycosylated.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession P07204 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • CD141
    • CD141 antigen
    • Fetomodulin
    • Thbd
    • THRM
    • Thrombomodulin
    • TM
    • TRBM_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

Thrombomodulin (CD141) Human ELISA Kit images

  • Representative Standard Curve using ab46508

Protocols

References for Thrombomodulin (CD141) Human ELISA Kit (ab46508)

This product has been referenced in:
  • Poursafa P  et al. The relationship of air pollution and surrogate markers of endothelial dysfunction in a population-based sample of children. BMC Public Health 11:115 (2011). Read more (PubMed: 21332998) »
  • Zhang G  et al. Constitutional flavonoids derived from Epimedium dose-dependently reduce incidence of steroid-associated osteonecrosis not via direct action by themselves on potential cellular targets. PLoS One 4:e6419 (2009). Read more (PubMed: 19641620) »

See all 2 Publications for this product

Product Wall

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The whole Thrombomodulin kit is stable if refrigerate (4°C) until at least for one year. However, w...

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I have just received an email from our collaborator. I am sorry I told you before I only summarized the complaints to our collaborator lab however I just realized I did have sent them the whole data for analysis. They...

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The following were the simple questions for which we never got reply;

- Does all the 5 kits show conc. range of 7ng/ml?
- I have checked the order history of this kit and found that the same item was bou...

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Thank you very much for your email. I am sorry to hear that you are experiencing problems with this product.

When we had chat on phone, last week I indeed said we recommend storing the standard at -20C however after further investigation, I ca...

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Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the ph...

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Thank you very much for your understanding. The things are more clearer now. Lab has confirmed that the number K100624 (typos K10624) is indeed stands for standard vial.

They have also confirmed that the standard conc. should be between 11-16...

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Thank you for confirming the details. I have sent your inquiry to my colleague in lab as I am unsure what this K110624 stands for. The standard bottle you received should not have any number so it will be interesting to know, what has happened. We do n...

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Could you please also tell me what is #K110624; this number seems unrelated to this kit?

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Thank you for contacting us.

We are happy to help. Could you please provide the catalogue number of the product? Could you explain what is K110624 this number seems unrelated to these kits?

I hope this information is helpful to yo...

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A panel of 40 human sera and 40 plasma samples were tested for CD141 in a recent batch. See results below

Sample Matrix Number of samples evaluated Range (ng/ml) Mean (ng/ml) Standard deviation (ng...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"