Anti-Thrombopoietin antibody (ab93909)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab93909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 38 kDa).

Target

  • FunctionLineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
  • Involvement in diseaseDefects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.
  • Sequence similaritiesBelongs to the EPO/TPO family.
  • DomainTwo-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C mpl ligand antibody
    • C-mpl ligand antibody
    • Megakaryocyte colony stimulating factor antibody
    • Megakaryocyte colony-stimulating factor antibody
    • Megakaryocyte growth and development factor antibody
    • Megakaryocyte stimulating factor antibody
    • MGC163194 antibody
    • MGDF antibody
    • MKCSF antibody
    • ML antibody
    • MPL ligand antibody
    • MPLLG antibody
    • Myeloproliferative leukemia virus oncogene ligand antibody
    • Prepro thrombopoietin antibody
    • THCYT1 antibody
    • THPO antibody
    • Thrombopoietin antibody
    • Thrombopoietin nirs variant 1 antibody
    • TPO antibody
    • TPO_HUMAN antibody
    see all

Anti-Thrombopoietin antibody images

  • Anti-Thrombopoietin antibody (ab93909) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute
  • ICC/IF image of ab93909 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab93909 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Thrombopoietin antibody (ab93909)

ab93909 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Human Cultured Cells (embryonic stem cells)
Specification embryonic stem cells
Permeabilization No
Fixative Methanol
Username

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Verified customer

Submitted Jun 07 2013

Thank you for contacting us.

Your credit note ID for the refund of both antibodies is xxx.

I am sorry that both antibodies did not perform as stated on their datasheets. I have asked our Finance department to issue a credit note for y...

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Thank you for your reply. I would still recommend checking if the antibodies bind to the recombinant protein in a dot blot assay. Because thrombopoietin is a secreted protein, it is very possible that it may be washed away from your cells during the st...

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Thank you for your reply. If I am understanding your assay correctly, your cells are overexpressing a thrombopoietin receptor and you are incubating the cell culture with recombinant thrombopoietin to measure the binding efficacy. What type of recombin...

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Thank you for your reply and the additional information.

Regarding the refund, you would need to let your purchasing department know the information I had sent and they then should contact us so that the refund can be issued to you. I'm certai...

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Thank you for contacting us.
I am sorry to hear that these antibodies do not work as expected.

According to the records in our system, the purchase of ab40664 has already been refunded and the case has been discussed and resolved.

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Thank you for your reply.

Only ab93909 has been tested with cultured cells (ICC/IF), while ab128487 has not yet been tested in this application. I can sendyou retroactively a testing discount code for trying it.

For UNTESTED specie...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"