Anti-Thrombopoietin antibody [BDI321] (ab31425)

Overview

• Product name
  Anti-Thrombopoietin antibody [BDI321]
  See all Thrombopoietin primary antibodies
• Description
  Mouse monoclonal [BDI321] to Thrombopoietin
• Specificity
  This antibody recognizes recombinant thrombopoietin: 100%: 0%; TPO (14–29): ~80%; rEPO: 0%; ACTH (1–39): 0%; Calcitonin: 0%; Insulin: 0%; Insulin like Growth Factor 1: 0%; Insulin like Growth Factor 2: 0%; Parathyroid hormone: 0%. The clone number has been updated from (1.B.6) to (BDI321) both clone numbers name the same antibody clone.
• Tested applications
  Suitable for: ELISA, WBmore details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Cow
• Immunogen

  Synthetic peptide:

  LTARLTLSSPAPPACD

  , corresponding to amino acids 14-29 of Human Thrombopoietin.

  Run BLAST with Run BLAST with

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage buffer
  Preservative: None
  Constituents: Tissue culture supernatant.
• Purity
  Tissue culture supernatant
• Clonality
  Monoclonal
• Clone number
  BDI321
• Isotype
  IgM
• Research areas
  • Cardiovascular
  • Blood
  • Fibrinolysis / Thrombolysis

  • Cell Biology
  • Cell Cycle
  • Cell differentiation

  • Stem Cells
  • Mesenchymal Stem Cells
  • Osteogenesis

  • Cancer
  • Cell cycle
  • Cell differentiation

Associated products

• Compatible Secondaries
  • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
  • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
  • Goat Anti-Mouse IgM H&L (ab9167)
  • Rabbit Anti-Mouse IgM mu chain (ab9175)

Applications

Target

• Function
  Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
• Involvement in disease
  Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.
• Sequence similarities
  Belongs to the EPO/TPO family.
• Domain
  Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.
• Cellular localization
  Secreted.

• Target information above from: UniProt accession P40225 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 7066 Human
  • Entrez Gene: 21832 Mouse
  • Entrez Gene: 81811 Rat
  • Omim: 600044 Human
  • SwissProt: P40225 Human
  • SwissProt: P40226 Mouse
  • SwissProt: P49745 Rat
  • Unigene: 1166 Human

  • Unigene: 3943 Mouse
  • Unigene: 10576 Rat

  see all
• Alternative names
  • C mpl ligand antibody
  • C-mpl ligand antibody
  • Megakaryocyte colony stimulating factor antibody

  • Megakaryocyte colony-stimulating factor antibody
  • Megakaryocyte growth and development factor antibody
  • Megakaryocyte stimulating factor antibody
  • MGC163194 antibody
  • MGDF antibody
  • MKCSF antibody
  • ML antibody
  • MPL ligand antibody
  • MPLLG antibody
  • Myeloproliferative leukemia virus oncogene ligand antibody
  • Prepro thrombopoietin antibody
  • THCYT1 antibody
  • THPO antibody
  • Thrombopoietin antibody
  • Thrombopoietin nirs variant 1 antibody
  • TPO antibody
  • TPO_HUMAN antibody

  see all

Protocols

• Western blot protocols