The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.25 - 1 µg/ml. Detection limit for recombinant Human Thrombopoietin: 0.2 - 0.4 ng/well.
Use a concentration of 0.25 - 1 µg/ml. Use as detection antibody with recommended pair. Detection of recombinant Human Thrombopoietin: 0.2 - 0.4 ng/well.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 38 kDa. Detection limit for recombinant Human Thrombopoietin: 1.5 - 3.0 ng/lane, under reducing or non-reducing conditions.
FunctionLineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Involvement in diseaseDefects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.
Sequence similaritiesBelongs to the EPO/TPO family.
DomainTwo-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.