Anti-Thrombospondin 2 antibody (ab84469)


  • Product nameAnti-Thrombospondin 2 antibody
    See all Thrombospondin 2 primary antibodies
  • Description
    Rabbit polyclonal to Thrombospondin 2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Horse, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human Thrombospondin 2.

    (Peptide available as ab96113.)

  • Positive control
    • This antibody gave a positive signal in both Human and Mouse spinal cord tissue lysate.


Associated products


Our Abpromise guarantee covers the use of ab84469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 129 kDa).
ICC/IF Use a concentration of 5 µg/ml.


Anti-Thrombospondin 2 antibody images

  • All lanes : Anti-Thrombospondin 2 antibody (ab84469) at 1 µg/ml

    Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 2 : Spinal Cord (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 129 kDa
    Observed band size : 56 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

    Human Thrombospondin-2 precursor detects a band at 58 kDa. While this differs to its predicted molecular weight of 129 kDa, this migration has been observed in the literature PMID:17082774 (ADAMTS1 mediates the release of antiangiogenic polypeptides from TSP1 and 2).

  • ICC/IF image of ab84469 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86649, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Thrombospondin 2 antibody (ab84469)

ab84469 has not yet been referenced specifically in any publications.

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