BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
Our Abpromise guarantee covers the use of ab1823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1-2µg for 106 cells.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
ab1823 - immunohistochemistry
Formalin fixed paraffin embedded human tonsil stained with Thrombospondin, using ABC and DAB chromogen.
ab1823 at a 1/25 dilution staining Human Glioblastoma Multiform tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the tissue was stained with the antibody for 1 hour. Bound antibody was detected using biotin anti-mouse probe .
This image is courtesy of an Abreview submitted by Francisca Wu on 14 March 2006.