Overview

  • Product nameAnti-Thrombospondin antibody [A6.1] (Biotin)
    See all Thrombospondin primary antibodies
  • Description
    Mouse monoclonal [A6.1] to Thrombospondin (Biotin)
  • ConjugationBiotin
  • Specificityab79450 shows no cross reaction with fibronectin, fibrinogen, and von Willebrand factor. It shows a mild cross reaction with Thrombospondin 2.
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, Electron Microscopy, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Horse, Cow, Dog, Human, Pig
  • Immunogen

    Reduced and alkylated purified human Thrombospondin (fully denatured) from the supernatant of thrombin activated platelets

  • EpitopeCollagen Type V binding domain of Thrombospondin
  • Positive control
    • Platelets in placenta, megakaryocytes in bone marrow and tonsil
  • General notes


    ab79450 inhibits Thrombospondin collagen interaction. Its binding to Thrombospondin is unaffected by glycosaminoglycans (e.g. hyaluronic acid, chondroitin sulfate, and heparin). Its binding is enhanced by EDTA i.e. at low concentration of Ca2+.

Properties

Applications

Our Abpromise guarantee covers the use of ab79450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 130 kDa.

For 2 hours at room temperature.

IP Use at 2 µg/mg of lysate.
IHC-P 1/25 - 1/50.
Flow Cyt Use at an assay dependent concentration. ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Electron Microscopy Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

References for Anti-Thrombospondin antibody [A6.1] (Biotin) (ab79450)

This product has been referenced in:
  • Galvin NJ  et al. Interaction of human thrombospondin with types I-V collagen: direct binding and electron microscopy. J Cell Biol 104:1413-22 (1987). Read more (PubMed: 3571333) »
  • Dixit VM  et al. Monoclonal antibodies that recognize calcium-dependent structures of human thrombospondin. Characterization and mapping of their epitopes. J Biol Chem 261:1962-8 (1986). Read more (PubMed: 2418018) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"