Overview

  • Product nameAnti-Thrombospondin antibody [A65M]
    See all Thrombospondin primary antibodies
  • Description
    Mouse monoclonal [A65M] to Thrombospondin
  • Specificityab23420 is highly specific for the low-calcium conformation of thrombospondin. There is no cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
  • Tested applicationsSuitable for: IP, IHC-Fr, ELISAmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Corneal endothelial cells on beads (Cow).

  • EpitopeEpitope is only present in thrombospondin prepared with low levels of calcium

Properties

Applications

Our Abpromise guarantee covers the use of ab23420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-Fr 1/40. PLP-fixed sections of bovine and human tissues.
ELISA 1/200.

Target

  • FunctionAdhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions.
  • Sequence similaritiesBelongs to the thrombospondin family.
    Contains 3 EGF-like domains.
    Contains 1 TSP C-terminal (TSPC) domain.
    Contains 1 TSP N-terminal (TSPN) domain.
    Contains 3 TSP type-1 domains.
    Contains 8 TSP type-3 repeats.
    Contains 1 VWFC domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • THBS 1 antibody
    • THBS antibody
    • Thbs1 antibody
    • Thrombospondin 1 antibody
    • Thrombospondin-1 antibody
    • TSP 1 antibody
    • TSP antibody
    • TSP1 antibody
    • TSP1_HUMAN antibody
    see all

References for Anti-Thrombospondin antibody [A65M] (ab23420)

This product has been referenced in:
  • He J & Bazan HE Epidermal growth factor synergism with TGF-beta1 via PI-3 kinase activity in corneal keratocyte differentiation. Invest Ophthalmol Vis Sci 49:2936-45 (2008). Read more (PubMed: 18579759) »
  • Behera MA  et al. Thrombospondin-1 and thrombospondin-2 mRNA and TSP-1 and TSP-2 protein expression in uterine fibroids and correlation to the genes COL1A1 and COL3A1 and to the collagen cross-link hydroxyproline. Reprod Sci 14:63-76 (2007). Read more (PubMed: 18089612) »

See all 2 Publications for this product

Product Wall

Thank you for your patience. I have been in correspondence with the source of each of the antibodies. Unfortunately PTP4A1 antibody (ab22825) is a fast track antibody and has not been applied to a positive control as such. ab23420 has not been ...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. PTP4A1 antibody (ab22825) is currently a fast track antibody that we cannot guarantee for the application of western blotting. We appreciat...

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Thank you for your enquiry. Ab23420 has only so far been tested for application in IHC-frozen tissue sections. It has not yet been tested with paraffin embedded sections. If you decide to go ahead and purchase this product, please let us know how yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"