• Product name
    Anti-Thrombospondin antibody [A65M]
    See all Thrombospondin primary antibodies
  • Description
    Mouse monoclonal [A65M] to Thrombospondin
  • Specificity
    ab23420 is highly specific for the low-calcium conformation of thrombospondin. There is no cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
  • Tested applications
    Suitable for: IP, IHC-Fr, ELISAmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Tissue, cells or virus corresponding to Cow Thrombospondin. Extracellular matrix material from cultured bovine corneal endothelial cells.

  • Epitope
    Epitope is only present in thrombospondin prepared in low-calcium (0.1 mmol/L) buffers.



Our Abpromise guarantee covers the use of ab23420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-Fr 1/40. PLP-fixed sections of bovine and human tissues.
ELISA 1/200.


  • Function
    Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions.
  • Sequence similarities
    Belongs to the thrombospondin family.
    Contains 3 EGF-like domains.
    Contains 1 TSP C-terminal (TSPC) domain.
    Contains 1 TSP N-terminal (TSPN) domain.
    Contains 3 TSP type-1 domains.
    Contains 8 TSP type-3 repeats.
    Contains 1 VWFC domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • THBS 1 antibody
    • THBS antibody
    • Thbs1 antibody
    • Thrombospondin 1 antibody
    • Thrombospondin-1 antibody
    • TSP 1 antibody
    • TSP antibody
    • TSP1 antibody
    • TSP1_HUMAN antibody
    see all

References for Anti-Thrombospondin antibody [A65M] (ab23420)

This product has been referenced in:
  • He J & Bazan HE Epidermal growth factor synergism with TGF-beta1 via PI-3 kinase activity in corneal keratocyte differentiation. Invest Ophthalmol Vis Sci 49:2936-45 (2008). Read more (PubMed: 18579759) »
  • Behera MA  et al. Thrombospondin-1 and thrombospondin-2 mRNA and TSP-1 and TSP-2 protein expression in uterine fibroids and correlation to the genes COL1A1 and COL3A1 and to the collagen cross-link hydroxyproline. Reprod Sci 14:63-76 (2007). Read more (PubMed: 18089612) »

See all 2 Publications for this product

Product Wall

Thank you for your patience. I have been in correspondence with the source of each of the antibodies. Unfortunately PTP4A1 antibody (ab22825) is a fast track antibody and has not been applied to a positive control as such. ab23420 has not been ...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. PTP4A1 antibody (ab22825) is currently a fast track antibody that we cannot guarantee for the application of western blotting. We appreciat...

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Thank you for your enquiry. Ab23420 has only so far been tested for application in IHC-frozen tissue sections. It has not yet been tested with paraffin embedded sections. If you decide to go ahead and purchase this product, please let us know how yo...

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