Overview

  • Product name
    Anti-Thrombospondin antibody
    See all Thrombospondin primary antibodies
  • Description
    Mouse polyclonal to Thrombospondin
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human Thrombospondin protein (NP_003237.2)

  • Positive control
    • WB: Human pancreas tissue lysate and Thrombospondin transfected 293T cell lysate IF: HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab88529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 129 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions.
  • Sequence similarities
    Belongs to the thrombospondin family.
    Contains 3 EGF-like domains.
    Contains 1 TSP C-terminal (TSPC) domain.
    Contains 1 TSP N-terminal (TSPN) domain.
    Contains 3 TSP type-1 domains.
    Contains 8 TSP type-3 repeats.
    Contains 1 VWFC domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • THBS 1 antibody
    • THBS antibody
    • Thbs1 antibody
    • Thrombospondin 1 antibody
    • Thrombospondin-1 antibody
    • TSP 1 antibody
    • TSP antibody
    • TSP1 antibody
    • TSP1_HUMAN antibody
    see all

Images

  • All lanes : Anti-Thrombospondin antibody (ab88529) at 1 µg/ml

    Lane 1 : Thrombospondin transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 129 kDa
  • ab88529 at 10 µg/ml staining Thrombospondin in paraformaldehyde fixed, permeabilised HeLa cells, by immunofluorescence.
  • Anti-Thrombospondin antibody (ab88529) at 1 µg/ml + Human pancreas tissue lysate at 50 µg

    Predicted band size : 129 kDa
    Observed band size : ~ 150 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Bonazzi VF  et al. Cross-Platform Array Screening Identifies COL1A2, THBS1, TNFRSF10D and UCHL1 as Genes Frequently Silenced by Methylation in Melanoma. PLoS One 6:e26121 (2011). WB ; Human . Read more (PubMed: 22028813) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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