Thrombospondin overexpression 293T lysate (whole cell) (ab94095)

Overview

  • Product nameThrombospondin overexpression 293T lysate (whole cell)
  • Description
    Thrombospondin overexpression lysate
  • General notesab94095 is a 293T cell transfected lysate in which Human Thrombospondin has been transiently over-expressed using a pCMV-Thrombospondin plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Similarity: Belongs to the thrombospondin family. Contains 3 EGF-like domains. Contains 1 TSP C-terminal (TSPC) domain. Contains 1 TSP N-terminal (TSPN) domain. Contains 3 TSP type-1 domains. Contains 8 TSP type-3 repeats. Contains 1 VWFC domain.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Thrombospondin overexpression 293T lysate (whole cell) images

  • ab94095 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Thrombospondin antibody (ab88529) at 1/500 dilution

    Lane 1 : Thrombospondin 293T Transfected Lysate - Positive Control (ab94095)
    Lane 2 : 293T Non Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Thrombospondin overexpression 293T lysate (whole cell) (ab94095)

ab94095 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"