Overview

  • Product name
    Anti-Thromboxane synthase antibody
    See all Thromboxane synthase primary antibodies
  • Description
    Rabbit polyclonal to Thromboxane synthase
  • Specificity
    This antibody is specific for Thromboxane synthase.
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC-FoFr, ICC, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    TNPDCQEKLLREVDVFKEK

    , corresponding to amino acids 359/377 of Human Thromboxane synthase

  • Positive control
    • Whole cell lysate from platelets.

Properties

Applications

Our Abpromise guarantee covers the use of ab39362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 61 kDa).Can be blocked with Human Thromboxane synthase peptide (ab72365).
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Tissue specificity
    Platelets, lung, kidney, spleen, macrophages and lung fibroblasts.
  • Involvement in disease
    Defects in TBXAS1 are the cause of Ghosal hematodiaphyseal dysplasia (GHDD) [MIM:231095]. GHDD is a rare autosomal recessive disorder characterized by increased bone density with predominant diaphyseal involvement and aregenerative corticosteroid-sensitive anemia. Aregenerative anemia is characterized by bone marrow failure, so that functional marrow cells are regenerated slowly or not at all.
    Defects in TBXAS1 are the cause of thromboxane synthetase deficiency (TBXAS1 deficiency) [MIM:274180]. It is characterized by hemorrhagic diathesis.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BDPLT14 antibody
    • CYP5 antibody
    • CYP5A1 antibody
    • Cytochrome P450 5A1 antibody
    • Cytochrome P450, family 5, subfamily A, polypeptide 1 antibody
    • FLJ52771 antibody
    • GHOSAL antibody
    • Platelet cytochrome P450 subfamily V antibody
    • Tbxas1 antibody
    • THAS antibody
    • THAS_HUMAN antibody
    • Thromboxane A synthase 1 (platelet) antibody
    • Thromboxane A synthase 1 (platelet, cytochrome P450, family 5, subfamily A) antibody
    • Thromboxane A synthase 1 antibody
    • Thromboxane A synthase 1 platelet cytochrome P450 subfamily V antibody
    • Thromboxane-A synthase antibody
    • TS antibody
    • TXA synthase antibody
    • TXAS antibody
    • TXS antibody
    see all

Anti-Thromboxane synthase antibody images

  • All lanes : Anti-Thromboxane synthase antibody (ab39362) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate - total protein (ab29699)
    Lane 2 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 21 kDa,36 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References for Anti-Thromboxane synthase antibody (ab39362)

This product has been referenced in:
  • Pai CH  et al. Lack of Thromboxane Synthase Prevents Hypertension and Fetal Growth Restriction after High Salt Treatment during Pregnancy. PLoS One 11:e0151617 (2016). IHC . Read more (PubMed: 26974824) »
  • Font-Nieves M  et al. Induction of COX-2 Enzyme and Down-regulation of COX-1 Expression by Lipopolysaccharide (LPS) Control Prostaglandin E2 Production in Astrocytes. J Biol Chem 287:6454-68 (2012). WB ; Mouse . Read more (PubMed: 22219191) »

See all 7 Publications for this product

Product Wall

Thank you for the additional information about your samples and protocol. I will be happy to issue a refund if you like. If you are interested, we also have another antibody which I recommend as a free replacement, ab68823:

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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