Anti-Thy1.1 antibody [HIS51] (Biotin) (ab95811)

Overview

  • Product name
    Anti-Thy1.1 antibody [HIS51] (Biotin)
    See all Thy1.1 primary antibodies
  • Description
    Mouse monoclonal [HIS51] to Thy1.1 (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Rat bone marrow cells

  • Positive control
    • Rat thymocyte and splenocyte suspensions

Properties

Applications

Our Abpromise guarantee covers the use of ab95811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.03-0.06µg for 105-8 cells. A final volume of 100 µL is recommended for staining the cell sample.



ab18454-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.
  • Cellular localization
    Attached to the membrane by a GPI anchor.
  • Database links
  • Alternative names
    • CD90 antibody
    • CD90.1 antibody
    • CDw90 antibody
    • Thy 1 cell surface antigen antibody
    • Thy 1 membrane glycoprotein antibody
    • Thy 1.1 antibody
    • Thy1 antibody
    • Thymus cell antigen 1, theta antibody
    see all

Anti-Thy1.1 antibody [HIS51] (Biotin) images

  • Flow cytometric analysis of Rat splenocytes with 0.03 µg of Anti-Mouse/Rat CD90.1 PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

References for Anti-Thy1.1 antibody [HIS51] (Biotin) (ab95811)

ab95811 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - whole (HEK and Mouse and Rat hippocapus)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
HEK and Mouse and Rat hippocapus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Submitted Apr 03 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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