Anti-Thy1.1 antibody [HIS51] (Biotin) (ab95811)

Overview

  • Product name
    Anti-Thy1.1 antibody [HIS51] (Biotin)
    See all Thy1.1 primary antibodies
  • Description
    Mouse monoclonal [HIS51] to Thy1.1 (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Rat bone marrow cells

  • Positive control
    • Rat thymocyte and splenocyte suspensions

Properties

Applications

Our Abpromise guarantee covers the use of ab95811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.03-0.06µg for 105-8 cells. A final volume of 100 µL is recommended for staining the cell sample.



ab18454-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.
  • Cellular localization
    Attached to the membrane by a GPI anchor.
  • Database links
  • Alternative names
    • CD90 antibody
    • CD90.1 antibody
    • CDw90 antibody
    • Thy 1 cell surface antigen antibody
    • Thy 1 membrane glycoprotein antibody
    • Thy 1.1 antibody
    • Thy1 antibody
    • Thymus cell antigen 1, theta antibody
    see all

Images

  • Flow cytometric analysis of Rat splenocytes with 0.03 µg of Anti-Mouse/Rat CD90.1 PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

References

ab95811 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (HEK and Mouse and Rat hippocapus)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
HEK and Mouse and Rat hippocapus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Verified customer

Submitted Apr 03 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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